Table 1

Quantities of uPA and other proteins in day-7 and day-13 QPD and control megakaryocyte cultures, evaluated by ELISA

Protein, unit/samplenMean ± SEM (range)/106 cells
Secreted
Cell associated
Day 7Day 13Day 13
uPA, pg 
    Q 460 ± 50 (320-550)* 16 000 ± 4,000 (7000-26 000)* 4700 ± 2000 (670-10 000)* 
    C 160 ± 80 (50-370) 150 ± 90 (40-440) 20 ± 7 (3-40) 
PAI-1, ng 
    Q < 0.05 63 ± 19 (31-109) 25 ± 7 (7-43) 
    C 1 ± 1 (<0.05-3) 68 ± 21 (16-105) 25 ± 8 (11-48) 
uPA-PAI-1 complexes, pg 
    Q < 100 500 ± 300 (< 100-1100) 600 ± 300 (< 100-1300) 
    C < 100 < 100 < 100 
PF-4, IU 
    Q < 0.3 148 ± 60 (27-210) 317 ± 146 (31-513) 
    C < 0.3 131 ± 46 (39-185) 245 ± 120 (46-460) 
TSP-1, % NPP 
    Q < 0.03 2.3 ± 0.6 (1.1-3.2) 2.2 ± 0.8 (0.6-3.1) 
    C < 0.03 3.4 ± 1.5 (0.8-6.0) 5.0 ± 2.2 (1.7-9.1) 
MMRN1, ng 
    Q < 25 25 ± 20 (< 25-147) 154 ± 36 (33-260) 
    C < 25 105 ± 39 (< 25-232) 255 ± 99 (75-761) 
VWF, ng 
    Q < 8 140 ± 36 (75-202) NE 
    C < 8 85 ± 36 (21-145) NE 
Protein, unit/samplenMean ± SEM (range)/106 cells
Secreted
Cell associated
Day 7Day 13Day 13
uPA, pg 
    Q 460 ± 50 (320-550)* 16 000 ± 4,000 (7000-26 000)* 4700 ± 2000 (670-10 000)* 
    C 160 ± 80 (50-370) 150 ± 90 (40-440) 20 ± 7 (3-40) 
PAI-1, ng 
    Q < 0.05 63 ± 19 (31-109) 25 ± 7 (7-43) 
    C 1 ± 1 (<0.05-3) 68 ± 21 (16-105) 25 ± 8 (11-48) 
uPA-PAI-1 complexes, pg 
    Q < 100 500 ± 300 (< 100-1100) 600 ± 300 (< 100-1300) 
    C < 100 < 100 < 100 
PF-4, IU 
    Q < 0.3 148 ± 60 (27-210) 317 ± 146 (31-513) 
    C < 0.3 131 ± 46 (39-185) 245 ± 120 (46-460) 
TSP-1, % NPP 
    Q < 0.03 2.3 ± 0.6 (1.1-3.2) 2.2 ± 0.8 (0.6-3.1) 
    C < 0.03 3.4 ± 1.5 (0.8-6.0) 5.0 ± 2.2 (1.7-9.1) 
MMRN1, ng 
    Q < 25 25 ± 20 (< 25-147) 154 ± 36 (33-260) 
    C < 25 105 ± 39 (< 25-232) 255 ± 99 (75-761) 
VWF, ng 
    Q < 8 140 ± 36 (75-202) NE 
    C < 8 85 ± 36 (21-145) NE 

Q indicates QPD; C, control; NPP, normal pooled platelets; and NE, not evaluated.

*

P < .05 for comparisons of Q versus C.

P < .05 for comparisons of day-13 versus day-7 samples.

Close Modal

or Create an Account

Close Modal
Close Modal