Neutrophils fail to respond to fMLP after interaction with activated platelets
| . | Mac-1, % basal value . | Neutrophils with internalized platelets, % . |
|---|---|---|
| Neutrophils with resting platelets | 100 | 0.3 ± 0.2 |
| Neutrophils with activated platelets | 158.6 ± 25.8 | 43.7 ± 5.1 |
| Neutrophils alone + fMLP | 242.8 ± 31.2 | 0.3 ± 0.2 |
| Neutrophils with resting platelets + fMLP | 246.2 ± 29.3 | 0.3 ± 0.2 |
| Neutrophils that phagocytosed activated platelets + fMLP | 126.5 ± 12.1 | 43.7 ± 5.1 |
| . | Mac-1, % basal value . | Neutrophils with internalized platelets, % . |
|---|---|---|
| Neutrophils with resting platelets | 100 | 0.3 ± 0.2 |
| Neutrophils with activated platelets | 158.6 ± 25.8 | 43.7 ± 5.1 |
| Neutrophils alone + fMLP | 242.8 ± 31.2 | 0.3 ± 0.2 |
| Neutrophils with resting platelets + fMLP | 246.2 ± 29.3 | 0.3 ± 0.2 |
| Neutrophils that phagocytosed activated platelets + fMLP | 126.5 ± 12.1 | 43.7 ± 5.1 |
Neutrophils were stimulated or not with fMLP 0.5 μM after a 5-minute incubation at 37°C with resting or activated platelets. Reactions were blocked after further 5 minutes. Mac-1 expression and internalized platelets were determined by flow cytometry. Effects on Mac-1 expression are expressed as the percentage of basal value. Values are the mean ± SEM of 3 independent experiments performed in duplicate.