Neutrophils that have internalized platelets circulate in the blood of patients with acute myocardial infarction
| . | Healthy subjects (n = 15) . | AMI patients (n = 15) . | P . |
|---|---|---|---|
| P-selectin, % of positive platelets | 7 ± 1 | 29 ± 3 | < .001 |
| Neutrophils MPO content, MFI | 60 ± 7 | 158 ± 8 | < .001 |
| Neutrophils with complete MPO depletion, % | 1.0 ± 0.5 | 31.2 ± 7.5 | < .001 |
| Neutrophils Mac-1 expression, MFI | 319 ± 20 | 556 ± 26 | < .001 |
| Neutrophils with adherent platelets, % | 7 ± 1 | 15 ± 2 | < .001 |
| Neutrophils with internalized platelets, % | 3 ± 1 | 16 ± 3 | < .001 |
| . | Healthy subjects (n = 15) . | AMI patients (n = 15) . | P . |
|---|---|---|---|
| P-selectin, % of positive platelets | 7 ± 1 | 29 ± 3 | < .001 |
| Neutrophils MPO content, MFI | 60 ± 7 | 158 ± 8 | < .001 |
| Neutrophils with complete MPO depletion, % | 1.0 ± 0.5 | 31.2 ± 7.5 | < .001 |
| Neutrophils Mac-1 expression, MFI | 319 ± 20 | 556 ± 26 | < .001 |
| Neutrophils with adherent platelets, % | 7 ± 1 | 15 ± 2 | < .001 |
| Neutrophils with internalized platelets, % | 3 ± 1 | 16 ± 3 | < .001 |
Platelet P-selectin expression, neutrophil myeloperoxidase content, Mac-1 expression (CD11b/CD18) on neutrophil surface, platelet-neutrophil aggregates and neutrophils with internalized platelets were determined in whole blood samples by 4-color flow cytometry as described in “Adhesion and phagocytosis.” Statistically significant differences were calculated by ANOVA and the Tukey test.
MFI indicates mean fluorescence intensity.