Mean telomere lengths and ultrashort telomeres in subpopulations of CB and MPB
| Subpopulation . | Mean . | n . | Outlier . | Mean, − outliers . | Mean . | n . | Outlier . | Mean, − outliers . |
|---|---|---|---|---|---|---|---|---|
| MPB-1 | MPB-2 | |||||||
| CD34+38−Rho− | 7.72 ± 0.17 | 231 | 0.09 | 8.21 ± 0.16 | — | — | — | — |
| CD34+38−Rho+ | 7.16 ± 0.20 | 245 | 0.13 | 7.93 ± 0.17 | 5.79 ± 0.21 | 173 | 0.03 | 5.96 ± 0.21 |
| CD34+CD38− | — | — | — | — | 5.23 ± 0.18 | 269 | 0.11 | 5.79 ± 0.19 |
| CD3+CD4+ | 6.29 ± 0.18 | 302 | 0.14 | 7.13 ± 0.16 | 5.29 ± 0.20 | 204 | 0.08 | 5.68 ± 0.20 |
| CD3+CD8+ | 6.13 ± 0.18 | 311 | 0.13 | 6.85 ± 0.16 | 5.14 ± 0.20 | 226 | 0.06 | 5.42 ± 0.20 |
| CD19/20+ | 7.10 ± 0.16 | 448 | 0.14 | 7.97 ± 0.15 | 6.33 ± 0.23 | 207 | 0.11 | 7.00 ± 0.21 |
| CD56+ | 5.50 ± 0.17 | 327 | 0.10 | 6.04 ± 0.16 | 4.32 ± 0.20 | 198 | 0.07 | 4.62 ± 0.20 |
| CD15/66b+ | 6.64 ± 0.19 | 264 | 0.17 | 7.71 ± 0.15 | — | — | — | — |
| MPB-3 | CB-1 | |||||||
| CD34+38−Rho− | 5.74 ± 0.28 | 118 | 0.06 | 6.07 ± 0.27 | — | — | — | — |
| CD34+38−Rho+ | 5.64 ± 0.29 | 85 | 0.07 | 5.99 ± 0.27 | 9.64 ± 0.65 | 39 | 0.10 | 10.54 ± 0.54 |
| CD34+CD38− | — | — | — | — | 8.55 ± 0.57 | 56 | 0.09 | 9.17 ± 0.55 |
| CD34+CD38+ | — | — | — | — | 8.16 ± 0.52 | 67 | 0.10 | 8.97 ± 0.48 |
| CD19/20+ | 5.53 ± 0.34 | 95 | 0.15 | 6.33 ± 0.32 | 7.66 ± 0.49 | 108 | 0.09 | 8.39 ± 0.48 |
| CD56+ | — | — | — | — | 6.51 ± 0.36 | 145 | 0.10 | 7.20 ± 0.38 |
| CD15/66b+ | 4.62 ± 0.32 | 76 | 0.20 | 5.54 ± 0.29 | — | — | — | — |
| CB-2 | CB-3 | |||||||
| CD34+38−Rho− | 9.20 ± 0.84 | 26 | 0.11 | 10.25 ± 0.68 | 9.90 ± 0.61 | 59 | 0.12 | 10.84 ± 0.58 |
| CD34+38−Rho+ | 8.24 ± 0.64 | 40 | 0.08 | 8.80 ± 0.60 | 7.95 ± 0.55 | 88 | ND | 7.95 ± 0.55 |
| CD34+CD38− | 7.63 ± 0.56 | 74 | 0.12 | 8.58 ± 0.53 | — | — | — | — |
| CD34+CD38+ | 7.55 ± 0.26 | 184 | 0.09 | 8.17 ± 0.24 | — | — | — | — |
| CD3+CD4+ | — | — | — | — | 6.85 ± 0.52 | 104 | ND | 6.85 ± 0.52 |
| CD3+CD8+ | — | — | — | — | 7.61 ± 0.50 | 87 | 0.10 | 8.41 ± 0.48 |
| CD19/20+ | 8.74 ± 0.42 | 108 | 0.14 | 9.84 ± 0.37 | 8.37 ± 0.76 | 48 | 0.17 | 9.86 ± 0.70 |
| CD56+ | 6.46 ± 0.32 | 123 | 0.11 | 7.19 ± 0.30 | 6.73 ± 0.66 | 56 | 0.02 | 6.85 ± 0.66 |
| CD15/66b+ | 7.10 ± 0.28 | 147 | 0.14 | 7.99 ± 0.23 | 7.10 ± 0.41 | 118 | 0.06 | 7.51 ± 0.40 |
| CB-4 | CB-4 after transplantation | |||||||
| CD34+38−Rho− | 11.28 ± 0.48 | 78 | 0.08 | 11.97 ± 0.43 | — | — | — | — |
| CD34+38−Rho+ | 10.85 ± 0.62 | 58 | 0.10 | 11.82 ± 0.55 | — | — | — | — |
| CD34+CD38+ | 10.01 ± 0.55 | 57 | 0.16 | 11.22 ± 0.48 | 7.32 ± 0.43 | 57 | 0.11 | 8.00 ± 0.38 |
| CD3+CD4+ | 9.70 ± 0.47 | 82 | 0.10 | 10.53 ± 0.42 | 6.15 ± 0.30 | 31 | 0.10 | 6.55 ± 0.21 |
| CD19/20+ | 10.96 ± 0.34 | 129 | 0.09 | 11.70 ± 0.29 | 7.28 ± 0.26 | 95 | 0.13 | 8.02 ± 0.19 |
| CD56+ | 8.35 ± 0.30 | 170 | 0.12 | 9.17 ± 0.28 | 5.27 ± 0.38 | 35 | 0.20 | 6.07 ± 0.32 |
| CD15/66b+ | 8.95 ± 0.34 | 113 | 0.07 | 9.46 ± 0.33 | 5.78 ± 0.34 | 59 | 0.19 | 6.85 ± 0.19 |
| CB-5 | CB-5 after transplantation | |||||||
| CD34+38−Rho− | 9.57 ± 0.52 | 50 | 0.08 | 10.11 ± 0.49 | — | — | — | — |
| CD34+38−Rho+ | 8.84 ± 0.48 | 64 | 0.09 | 9.48 ± 0.45 | — | — | — | — |
| CD34+CD38− | 8.60 ± 0.28 | 167 | 0.12 | 9.38 ± 0.27 | — | — | — | — |
| CD34+CD38+ | 7.87 ± 0.57 | 48 | 0.08 | 8.46 ± 0.54 | 6.62 ± 0.21 | 131 | 0.14 | 7.32 ± 0.16 |
| CD19/20+ | 7.39 ± 0.51 | 67 | 0.13 | 8.37 ± 0.47 | 7.58 ± 0.32 | 101 | 0.17 | 8.67 ± 0.24 |
| CD56+ | 5.82 ± 0.48 | 52 | 0.15 | 6.74 ± 0.44 | 4.78 ± 0.46 | 27 | 0.19 | 5.59 ± 0.38 |
| CD15/66b+ | 6.29 ± 0.48 | 58 | 0.12 | 7.05 ± 0.45 | 4.90 ± 0.19 | 125 | 0.15 | 5.68 ± 0.13 |
| CB-6 | CB-6 mouse 1 | |||||||
| CD34+CD38+ | 9.10 ± 0.23 | 264 | 0.10 | 9.92 ± 0.20 | 6.61 ± 0.20 | 163 | 0.15 | 7.32 ± 0.18 |
| CD3+CD4+ | 7.71 ± 0.19 | 284 | 0.07 | 8.19 ± 0.17 | 5.30 ± 0.26 | 75 | 0.16 | 6.01 ± 0.21 |
| CD15/66b+ | 6.96 ± 0.25 | 188 | 0.12 | 7.68 ± 0.24 | 4.96 ± 0.21 | 99 | 0.22 | 5.76 ± 0.14 |
| CB-6 mouse 2 | CB-6 mouse 3 | |||||||
| CD34+CD38+ | 6.58 ± 0.21 | 143 | 0.13 | 7.24 ± 0.18 | 6.35 ± 0.23 | 168 | 0.13 | 7.12 ± 0.19 |
| CD3+CD4+ | 5.23 ± 0.20 | 100 | 0.11 | 5.67 ± 0.17 | 5.35 ± 0.17 | 148 | 0.18 | 6.06 ± 0.14 |
| CD15/66b+ | 5.38 ± 0.29 | 54 | 0.13 | 5.99 ± 0.21 | 4.93 ± 0.21 | 113 | 0.16 | 5.66 ± 0.15 |
| Subpopulation . | Mean . | n . | Outlier . | Mean, − outliers . | Mean . | n . | Outlier . | Mean, − outliers . |
|---|---|---|---|---|---|---|---|---|
| MPB-1 | MPB-2 | |||||||
| CD34+38−Rho− | 7.72 ± 0.17 | 231 | 0.09 | 8.21 ± 0.16 | — | — | — | — |
| CD34+38−Rho+ | 7.16 ± 0.20 | 245 | 0.13 | 7.93 ± 0.17 | 5.79 ± 0.21 | 173 | 0.03 | 5.96 ± 0.21 |
| CD34+CD38− | — | — | — | — | 5.23 ± 0.18 | 269 | 0.11 | 5.79 ± 0.19 |
| CD3+CD4+ | 6.29 ± 0.18 | 302 | 0.14 | 7.13 ± 0.16 | 5.29 ± 0.20 | 204 | 0.08 | 5.68 ± 0.20 |
| CD3+CD8+ | 6.13 ± 0.18 | 311 | 0.13 | 6.85 ± 0.16 | 5.14 ± 0.20 | 226 | 0.06 | 5.42 ± 0.20 |
| CD19/20+ | 7.10 ± 0.16 | 448 | 0.14 | 7.97 ± 0.15 | 6.33 ± 0.23 | 207 | 0.11 | 7.00 ± 0.21 |
| CD56+ | 5.50 ± 0.17 | 327 | 0.10 | 6.04 ± 0.16 | 4.32 ± 0.20 | 198 | 0.07 | 4.62 ± 0.20 |
| CD15/66b+ | 6.64 ± 0.19 | 264 | 0.17 | 7.71 ± 0.15 | — | — | — | — |
| MPB-3 | CB-1 | |||||||
| CD34+38−Rho− | 5.74 ± 0.28 | 118 | 0.06 | 6.07 ± 0.27 | — | — | — | — |
| CD34+38−Rho+ | 5.64 ± 0.29 | 85 | 0.07 | 5.99 ± 0.27 | 9.64 ± 0.65 | 39 | 0.10 | 10.54 ± 0.54 |
| CD34+CD38− | — | — | — | — | 8.55 ± 0.57 | 56 | 0.09 | 9.17 ± 0.55 |
| CD34+CD38+ | — | — | — | — | 8.16 ± 0.52 | 67 | 0.10 | 8.97 ± 0.48 |
| CD19/20+ | 5.53 ± 0.34 | 95 | 0.15 | 6.33 ± 0.32 | 7.66 ± 0.49 | 108 | 0.09 | 8.39 ± 0.48 |
| CD56+ | — | — | — | — | 6.51 ± 0.36 | 145 | 0.10 | 7.20 ± 0.38 |
| CD15/66b+ | 4.62 ± 0.32 | 76 | 0.20 | 5.54 ± 0.29 | — | — | — | — |
| CB-2 | CB-3 | |||||||
| CD34+38−Rho− | 9.20 ± 0.84 | 26 | 0.11 | 10.25 ± 0.68 | 9.90 ± 0.61 | 59 | 0.12 | 10.84 ± 0.58 |
| CD34+38−Rho+ | 8.24 ± 0.64 | 40 | 0.08 | 8.80 ± 0.60 | 7.95 ± 0.55 | 88 | ND | 7.95 ± 0.55 |
| CD34+CD38− | 7.63 ± 0.56 | 74 | 0.12 | 8.58 ± 0.53 | — | — | — | — |
| CD34+CD38+ | 7.55 ± 0.26 | 184 | 0.09 | 8.17 ± 0.24 | — | — | — | — |
| CD3+CD4+ | — | — | — | — | 6.85 ± 0.52 | 104 | ND | 6.85 ± 0.52 |
| CD3+CD8+ | — | — | — | — | 7.61 ± 0.50 | 87 | 0.10 | 8.41 ± 0.48 |
| CD19/20+ | 8.74 ± 0.42 | 108 | 0.14 | 9.84 ± 0.37 | 8.37 ± 0.76 | 48 | 0.17 | 9.86 ± 0.70 |
| CD56+ | 6.46 ± 0.32 | 123 | 0.11 | 7.19 ± 0.30 | 6.73 ± 0.66 | 56 | 0.02 | 6.85 ± 0.66 |
| CD15/66b+ | 7.10 ± 0.28 | 147 | 0.14 | 7.99 ± 0.23 | 7.10 ± 0.41 | 118 | 0.06 | 7.51 ± 0.40 |
| CB-4 | CB-4 after transplantation | |||||||
| CD34+38−Rho− | 11.28 ± 0.48 | 78 | 0.08 | 11.97 ± 0.43 | — | — | — | — |
| CD34+38−Rho+ | 10.85 ± 0.62 | 58 | 0.10 | 11.82 ± 0.55 | — | — | — | — |
| CD34+CD38+ | 10.01 ± 0.55 | 57 | 0.16 | 11.22 ± 0.48 | 7.32 ± 0.43 | 57 | 0.11 | 8.00 ± 0.38 |
| CD3+CD4+ | 9.70 ± 0.47 | 82 | 0.10 | 10.53 ± 0.42 | 6.15 ± 0.30 | 31 | 0.10 | 6.55 ± 0.21 |
| CD19/20+ | 10.96 ± 0.34 | 129 | 0.09 | 11.70 ± 0.29 | 7.28 ± 0.26 | 95 | 0.13 | 8.02 ± 0.19 |
| CD56+ | 8.35 ± 0.30 | 170 | 0.12 | 9.17 ± 0.28 | 5.27 ± 0.38 | 35 | 0.20 | 6.07 ± 0.32 |
| CD15/66b+ | 8.95 ± 0.34 | 113 | 0.07 | 9.46 ± 0.33 | 5.78 ± 0.34 | 59 | 0.19 | 6.85 ± 0.19 |
| CB-5 | CB-5 after transplantation | |||||||
| CD34+38−Rho− | 9.57 ± 0.52 | 50 | 0.08 | 10.11 ± 0.49 | — | — | — | — |
| CD34+38−Rho+ | 8.84 ± 0.48 | 64 | 0.09 | 9.48 ± 0.45 | — | — | — | — |
| CD34+CD38− | 8.60 ± 0.28 | 167 | 0.12 | 9.38 ± 0.27 | — | — | — | — |
| CD34+CD38+ | 7.87 ± 0.57 | 48 | 0.08 | 8.46 ± 0.54 | 6.62 ± 0.21 | 131 | 0.14 | 7.32 ± 0.16 |
| CD19/20+ | 7.39 ± 0.51 | 67 | 0.13 | 8.37 ± 0.47 | 7.58 ± 0.32 | 101 | 0.17 | 8.67 ± 0.24 |
| CD56+ | 5.82 ± 0.48 | 52 | 0.15 | 6.74 ± 0.44 | 4.78 ± 0.46 | 27 | 0.19 | 5.59 ± 0.38 |
| CD15/66b+ | 6.29 ± 0.48 | 58 | 0.12 | 7.05 ± 0.45 | 4.90 ± 0.19 | 125 | 0.15 | 5.68 ± 0.13 |
| CB-6 | CB-6 mouse 1 | |||||||
| CD34+CD38+ | 9.10 ± 0.23 | 264 | 0.10 | 9.92 ± 0.20 | 6.61 ± 0.20 | 163 | 0.15 | 7.32 ± 0.18 |
| CD3+CD4+ | 7.71 ± 0.19 | 284 | 0.07 | 8.19 ± 0.17 | 5.30 ± 0.26 | 75 | 0.16 | 6.01 ± 0.21 |
| CD15/66b+ | 6.96 ± 0.25 | 188 | 0.12 | 7.68 ± 0.24 | 4.96 ± 0.21 | 99 | 0.22 | 5.76 ± 0.14 |
| CB-6 mouse 2 | CB-6 mouse 3 | |||||||
| CD34+CD38+ | 6.58 ± 0.21 | 143 | 0.13 | 7.24 ± 0.18 | 6.35 ± 0.23 | 168 | 0.13 | 7.12 ± 0.19 |
| CD3+CD4+ | 5.23 ± 0.20 | 100 | 0.11 | 5.67 ± 0.17 | 5.35 ± 0.17 | 148 | 0.18 | 6.06 ± 0.14 |
| CD15/66b+ | 5.38 ± 0.29 | 54 | 0.13 | 5.99 ± 0.21 | 4.93 ± 0.21 | 113 | 0.16 | 5.66 ± 0.15 |
Data are represented as mean telomere lengths (± SEM) of each subpopulation in the 3 MPB and 6 CB studied. The number of XpYp telomeres scored (n), and frequency of outliers, representing telomeres that are likely to have shortened by processes other than normal replication, are also shown. Measures of mean telomere length (± SEM) with the outliers excluded are also given, and are used in subsequent analyses where the number of divisions is inferred from the changes in telomere length.
— indicates no reliable data.