Expansion of MSCs in culture
| Days in culture . | Donor 1, ×1000 cells/cm2 . | Donor 3, ×1000 cells/cm2 . | Donor 4, ×1000 cells/cm2 . | Donor 5, ×1000 cells/cm2 . |
|---|---|---|---|---|
| 5 | 3.4 ± 0.5 | 3.6 ± 1.1 | 4.6 ± 0.6 | 6.2 ± 0.5* |
| 6 | 6.4 ± 0.8 | 5.7 ± 0.5 | 10.3 ± 1.6 | 15.1 ± 2.1 |
| 7 | 15.6 ± 2.2 | 16.2 ± 3.6 | 20.1 ± 1.6 | 30.2 ± 7.9 |
| 8 | 30.5 ± 5.6 | > 19.0† | > 42.0† | 42.9 ± 5.7 |
| 9 | > 50.0† | > 26.0† | > 52.0† | > 57.0† |
| Days in culture . | Donor 1, ×1000 cells/cm2 . | Donor 3, ×1000 cells/cm2 . | Donor 4, ×1000 cells/cm2 . | Donor 5, ×1000 cells/cm2 . |
|---|---|---|---|---|
| 5 | 3.4 ± 0.5 | 3.6 ± 1.1 | 4.6 ± 0.6 | 6.2 ± 0.5* |
| 6 | 6.4 ± 0.8 | 5.7 ± 0.5 | 10.3 ± 1.6 | 15.1 ± 2.1 |
| 7 | 15.6 ± 2.2 | 16.2 ± 3.6 | 20.1 ± 1.6 | 30.2 ± 7.9 |
| 8 | 30.5 ± 5.6 | > 19.0† | > 42.0† | 42.9 ± 5.7 |
| 9 | > 50.0† | > 26.0† | > 52.0† | > 57.0† |
Frozen vials containing approximately 106 passage 1 MSCs from 5 donors were thawed, plated in 174-cm2 dishes, incubated for 1 day, replated at 100 cells/cm2 on the next day, and cultured for 5 to 9 days. Confluency was estimated by phase microscopy of the cultures and cell density by counting with a hemocytometer of cultures lifted with trypsin/EDTA.
EDTA indicates ethylenediaminetetraacetic acid; and MSC, multipotent stromal cells.
Values are mean plus or minus SD (n = 5).
Cells aggregated.