Summary of the LAM-PCR sequencing results
| Liver sample . | Sequence . | Junction . |
|---|---|---|
| Brad | Human α1-antitrypsin promoter region (118 bp) | 5′ end of transcription start as present in vector |
| Brad | Canine FIX cDNA (64 bp) | 3′-untranslated region as present in vector |
| Brad | Human β-globin (34 bp) | Exon/intron splice junction as present in vector |
| Brad | Canine FIX cDNA (64 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (143 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (105 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (153 bp) | 3′-untranslated region as present in vector |
| Brad | AAV vector (43 bp) | Part of expression cassette |
| Brad | ApoE enhancer (135 bp) | Enhancer used in expression cassette |
| Brad | AAV vector (42 bp) | Cloning sites |
| Brad | AAV vector (40 bp) | Cloning sites |
| Brad | hGH poly(A) (131 bp) | Poly(A) used in AAV vector cassette |
| Semillon | AAV vector (222 bp) | hGH poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (98 bp) | 3′-untranslated region as present in vector |
| Semillon | AAV vector (47 bp) | Cloning sites |
| Semillon | hGH poly(A) (177 bp) | Poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (144 bp) | 3′-untranslated region as present in vector |
| Semillon | Canine FIX cDNA (143 bp) | 3′-untranslated region as present in vector |
| Semillon | hGH poly(A) (121 bp) | Poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (32 bp) | 3′-untranslated region as present in vector |
| Semillon | Plasmid DNA (106 bp) | (Packaging of helper plasmid DNA or cloning artifact) |
| Liver sample . | Sequence . | Junction . |
|---|---|---|
| Brad | Human α1-antitrypsin promoter region (118 bp) | 5′ end of transcription start as present in vector |
| Brad | Canine FIX cDNA (64 bp) | 3′-untranslated region as present in vector |
| Brad | Human β-globin (34 bp) | Exon/intron splice junction as present in vector |
| Brad | Canine FIX cDNA (64 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (143 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (105 bp) | 3′-untranslated region as present in vector |
| Brad | Canine FIX cDNA (153 bp) | 3′-untranslated region as present in vector |
| Brad | AAV vector (43 bp) | Part of expression cassette |
| Brad | ApoE enhancer (135 bp) | Enhancer used in expression cassette |
| Brad | AAV vector (42 bp) | Cloning sites |
| Brad | AAV vector (40 bp) | Cloning sites |
| Brad | hGH poly(A) (131 bp) | Poly(A) used in AAV vector cassette |
| Semillon | AAV vector (222 bp) | hGH poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (98 bp) | 3′-untranslated region as present in vector |
| Semillon | AAV vector (47 bp) | Cloning sites |
| Semillon | hGH poly(A) (177 bp) | Poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (144 bp) | 3′-untranslated region as present in vector |
| Semillon | Canine FIX cDNA (143 bp) | 3′-untranslated region as present in vector |
| Semillon | hGH poly(A) (121 bp) | Poly(A) used in AAV vector cassette |
| Semillon | Canine FIX cDNA (32 bp) | 3′-untranslated region as present in vector |
| Semillon | Plasmid DNA (106 bp) | (Packaging of helper plasmid DNA or cloning artifact) |