The stimulus-dependent role of endothelial cell junctional molecules in mediating cytokine-induced leukocyte transmigration is not governed by the mouse strain used
| Blocking antibody . | Percentage reduction in IL-1β–stimulated TEM . | Percentage reduction in TNF-α–stimulated TEM . | ||||
|---|---|---|---|---|---|---|
| C57BL/6 . | FVB/n . | SJL . | C57BL/6 . | FVB/n . | SJL . | |
| Anti–ICAM-2 | 48.4 ± 6.9* | 80.05 ± 7.1† | 49.95 ± 21.2 | −0.002 ± 6.8 | −1.04 ± 12.5 | −3.1 ± 20.2 |
| Anti–JAM-A | 62.2 ± 10.8‡ | 69.3 ± 5.6* | 66.7 ± 18.4* | −16.8 ± 17 | −5.98 ± 38.6 | −4.8 ± 29.9 |
| Anti–PECAM-1 | 43.1 ± 10.7† | 73.7 ± 34.1* | 78.3 ± 14.4† | −23.6 ± 5.2 | −15.59 ± 41.3 | 15.9 ± 20.3 |
| Blocking antibody . | Percentage reduction in IL-1β–stimulated TEM . | Percentage reduction in TNF-α–stimulated TEM . | ||||
|---|---|---|---|---|---|---|
| C57BL/6 . | FVB/n . | SJL . | C57BL/6 . | FVB/n . | SJL . | |
| Anti–ICAM-2 | 48.4 ± 6.9* | 80.05 ± 7.1† | 49.95 ± 21.2 | −0.002 ± 6.8 | −1.04 ± 12.5 | −3.1 ± 20.2 |
| Anti–JAM-A | 62.2 ± 10.8‡ | 69.3 ± 5.6* | 66.7 ± 18.4* | −16.8 ± 17 | −5.98 ± 38.6 | −4.8 ± 29.9 |
| Anti–PECAM-1 | 43.1 ± 10.7† | 73.7 ± 34.1* | 78.3 ± 14.4† | −23.6 ± 5.2 | −15.59 ± 41.3 | 15.9 ± 20.3 |
Three mouse strains, C57BL/6, FVB/n, and SJL, were used in studies of leukocyte transmigration through IL-1β (50 ng)– and TNF-α (300 ng)–stimulated cremasteric venules. Mice were pretreated with blocking mAbs directed against ICAM-2, JAM-A, PECAM-1, or appropriate control mAbs (all at 3 mg/kg intravenously) before local injection of cytokines. After 4 hours, leukocyte transmigration was quantified by intravital microscopy, as detailed in “Intravital microscopy.” Data show percentage reduction in number of transmigrated leukocytes in a 10−4 μm2 area of perivascular tissue in the different mouse strains and as treated with different blocking mAbs compared with control antibody-treated animals of the same strain (negative numbers indicate small and insignificant increases in responses). Results are mean ± SEM from n = 3 to 7 mice/group.
Significant reduction (P < .05).
Significant reduction (P < .01).
Significant reduction (P < .001).