Microscopic and macroscopic diffusion data of healthy and disease RBCs
| RBC type . | Dμ, cm2/s . | DM, cm2/s . | Percent immobile* . | Percent constrained† . | Percent free . |
|---|---|---|---|---|---|
| Fixed (n = 134)‡ | (6.8 ± 0.1) × 10−14§ | (5.5 ± 0.2) × 10−14 | 95‖ | 0 | 5 |
| Normal (n = 626) | (1.4 ± 0.4) × 10−11 (77%)¶(2.2 ± 0.1) × 10−10 (23%) | (1.7 ± 0.1) × 10−12 | 24 | 30 | 46 |
| HPP (n = 349) | (7.9 ± 0.5) × 10−11 | (1.5 ± 0.1) × 10−11 | 7 | 37 | 56 |
| HS-1 (n = 107) | (2.7 ± 1.3) × 10−12 (27%)(5.5 ± 0.7) × 10−11 (73%) | (1.4 ± 0.1) × 10−12 | 34 | 32 | 34 |
| HS-2 (n = 124) | (3.8 ± 1.1) × 10−12 (42%)(9.6 ± 1.2) × 10−11 (58%) | (2.2 ± 0.1) × 10−12 | 20 | 37 | 43 |
| HE-1 (n = 133) | (5.4 ± 0.8) × 10−11 | (9.8 ± 1.7) × 10−13 (69%)(2.0 ± 0.4) × 10−10 (31%) | 23 | 31 | 46 |
| HE-2 (n = 212) | (1.2 ± 0.1) × 10−12 (37%)(3.0 ± 0.3) × 10−11 (63%) | (8.8 ± 0.5) × 10−13 | 19 | 25 | 56 |
| HE-3 (n = 168) | (8.2 ± 0.4) × 10−13 (20%)(2.6 ± 0.4) × 10−11 (80%) | (8.3 ± 0.3) × 10−13 (78%)(8.1 ± 1.1) × 10−11 (22%) | 26 | 22 | 52 |
| HHy (n = 171) | (1.5 ± 1.2) × 10−12 (40%)(4.4 ± 1.4) × 10−11 (60%) | (8.1 ± 0.4) × 10−13 | 31 | 30 | 39 |
| SAO (n = 173) | (2.1 ± 0.1) × 10−12 (16%)(4.5 ± 0.3) × 10−11 (84%) | (1.8 ± 0.1) × 10−12 | 21 | 31 | 48 |
| HS-unknown defects | |||||
| HS-3 (n = 231) | (1.2 ± 1.9) × 10−12 (25%)(2.9 ± 0.3) × 10−11 (75%) | (6.8 ± 0.3) × 10−12 | 34 | 28 | 38 |
| HS-4 (n = 173) | (4.9 ± 0.3) × 10−11 | (4.0 ± 0.2) × 10−13 | 21 | 33 | 46 |
| HS-5 (n = 206) | (1.8 ± 0.3) × 10−12 (17%)(6.7 ± 0.4) × 10−11 (82%) | (1.8 ± 0.1) × 10−12 | 25 | 35 | 40 |
| HS-6 (n = 136) | (2.7 ± 0.5) × 10−12 (11%)(1.0 ± 0.2) × 10−10 (89%) | (3.2 ± 0.1) × 10−12 | 20 | 30 | 50 |
| RBC type . | Dμ, cm2/s . | DM, cm2/s . | Percent immobile* . | Percent constrained† . | Percent free . |
|---|---|---|---|---|---|
| Fixed (n = 134)‡ | (6.8 ± 0.1) × 10−14§ | (5.5 ± 0.2) × 10−14 | 95‖ | 0 | 5 |
| Normal (n = 626) | (1.4 ± 0.4) × 10−11 (77%)¶(2.2 ± 0.1) × 10−10 (23%) | (1.7 ± 0.1) × 10−12 | 24 | 30 | 46 |
| HPP (n = 349) | (7.9 ± 0.5) × 10−11 | (1.5 ± 0.1) × 10−11 | 7 | 37 | 56 |
| HS-1 (n = 107) | (2.7 ± 1.3) × 10−12 (27%)(5.5 ± 0.7) × 10−11 (73%) | (1.4 ± 0.1) × 10−12 | 34 | 32 | 34 |
| HS-2 (n = 124) | (3.8 ± 1.1) × 10−12 (42%)(9.6 ± 1.2) × 10−11 (58%) | (2.2 ± 0.1) × 10−12 | 20 | 37 | 43 |
| HE-1 (n = 133) | (5.4 ± 0.8) × 10−11 | (9.8 ± 1.7) × 10−13 (69%)(2.0 ± 0.4) × 10−10 (31%) | 23 | 31 | 46 |
| HE-2 (n = 212) | (1.2 ± 0.1) × 10−12 (37%)(3.0 ± 0.3) × 10−11 (63%) | (8.8 ± 0.5) × 10−13 | 19 | 25 | 56 |
| HE-3 (n = 168) | (8.2 ± 0.4) × 10−13 (20%)(2.6 ± 0.4) × 10−11 (80%) | (8.3 ± 0.3) × 10−13 (78%)(8.1 ± 1.1) × 10−11 (22%) | 26 | 22 | 52 |
| HHy (n = 171) | (1.5 ± 1.2) × 10−12 (40%)(4.4 ± 1.4) × 10−11 (60%) | (8.1 ± 0.4) × 10−13 | 31 | 30 | 39 |
| SAO (n = 173) | (2.1 ± 0.1) × 10−12 (16%)(4.5 ± 0.3) × 10−11 (84%) | (1.8 ± 0.1) × 10−12 | 21 | 31 | 48 |
| HS-unknown defects | |||||
| HS-3 (n = 231) | (1.2 ± 1.9) × 10−12 (25%)(2.9 ± 0.3) × 10−11 (75%) | (6.8 ± 0.3) × 10−12 | 34 | 28 | 38 |
| HS-4 (n = 173) | (4.9 ± 0.3) × 10−11 | (4.0 ± 0.2) × 10−13 | 21 | 33 | 46 |
| HS-5 (n = 206) | (1.8 ± 0.3) × 10−12 (17%)(6.7 ± 0.4) × 10−11 (82%) | (1.8 ± 0.1) × 10−12 | 25 | 35 | 40 |
| HS-6 (n = 136) | (2.7 ± 0.5) × 10−12 (11%)(1.0 ± 0.2) × 10−10 (89%) | (3.2 ± 0.1) × 10−12 | 20 | 30 | 50 |
The totally immobile fraction is defined as the fraction of band 3 molecules that was found to have a DM value less than or equal to the slowest 95% of DM values measured for band 3 on fixed erythrocytes (DMImm); that is, the fraction of band 3 that diffuses similar to band 3 on fixed cells.
Percent confined includes all trajectories that were classified as undergoing confined or hop diffusion and for which DM > DMImm.
Total number of trajectories analyzed.
Errors are given as the SEM.
Defined as such.
Reflects the fraction of total area contained in each peak of the Gaussian fit to the distribution. For those distributions that fit best with a single peak it is understood that 100% of the area is contained within that peak