Table 1 Kinetic analysis of wild-type... | American Society of Hematology

Table 1

Kinetic analysis of wild-type ADAMTS13 and mutant D187A ADAMTS13 proteolysis of VWF115

ADAMTS13	Time course (n = 3), k_cat/K_m, 10⁶ M⁻¹s⁻¹	Michaelis-Menten
ADAMTS13	Time course (n = 3), k_cat/K_m, 10⁶ M⁻¹s⁻¹	K_m, μM	k_cat, s⁻¹	k_cat/K_m, 10⁶ M⁻¹s⁻¹
Wild-type	1.24 ± 0.10	1.29	1.31	1.02
D187A	0.10 ± 0.02	4.32	0.31	0.07

ADAMTS13	Time course (n = 3), k_cat/K_m, 10⁶ M⁻¹s⁻¹	Michaelis-Menten
ADAMTS13	Time course (n = 3), k_cat/K_m, 10⁶ M⁻¹s⁻¹	K_m, μM	k_cat, s⁻¹	k_cat/K_m, 10⁶ M⁻¹s⁻¹
Wild-type	1.24 ± 0.10	1.29	1.31	1.02
D187A	0.10 ± 0.02	4.32	0.31	0.07