Results of antibody microarray screening
| Target protein name . | Phospho site (human) . | % CFC . |
|---|---|---|
| 4E-BP1 | S65 | −378 |
| Aik | Pan-specific | −55 |
| ALS2CR7[PFTAIRE2] | Pan-specific | −64 |
| Axl | Pan-specific | −81 |
| B23 (NPM) | T199 | 78 |
| CASP2 | Pan-specific | −55 |
| CREB1 | S129 + S133 | 85 |
| CREB1 | S133 | 56 |
| EGFR | Y1068 | 75 |
| eIF2Be | S540 | 57 |
| eIF4E | S209 | 93 |
| ErbB2[HER2] | Y1248 | 63 |
| Erk1/2 | T202 + Y204 | 83 |
| Erk5 | T218 + Y220 | 62 |
| FAK | S732 | 56 |
| Hsp25 | S86 | 53 |
| IGF1R | Pan-specific | −57 |
| Integrin a4 | S988 | 59 |
| IRAK4 | Pan-specific | −52 |
| JNK2 | Pan-specific | −82 |
| Jun | S73 | −83 |
| Kit | Y703 | 53 |
| MAPKAPK2 | T334 | 69 |
| Mcl-1 | Pan-specific | 675 |
| MEKK2[MAP3K2] | Pan-specific | −56 |
| MRLC2 | S18 | 66 |
| p16INK4 | Pan-specific | 77 |
| p38aMAPK | T180 + Y182 | 151 |
| PDGFRb | Y716 | 84 |
| PKA Ca/b | T197 | 70 |
| PKA R2a | S98 | −61 |
| PKBa (Akt1) | Pan-specific | 63 |
| PKBg (Akt3) | Pan-specific | −60 |
| PKCg | Pan-specific | 98 |
| PKCh | T655 | 51 |
| PKCq | S695 | 66 |
| PKCz | Pan-specific | 250 |
| PKR1 | Pan-specific | 51 |
| PKR1 | T451 | 66 |
| Plk1 | T210 | 73 |
| Plk2 | Pan-specific | 61 |
| PP1/Ca | T320 | 128 |
| PP2Cd | Pan-specific | 59 |
| PP4C | Pan-specific | 64 |
| PP5C | Pan-specific | 67 |
| PRK2[PKN2] | Pan-specific | 69 |
| PTEN | S370 | 121 |
| PTP1B | Pan-specific | 51 |
| Pyk2 | Pan-specific | 56 |
| Rac1/cdc42 | S71 | 52 |
| Rb | Pan-specific | 167 |
| Rb | S612 | 131 |
| Rb | S807 | 102 |
| Rb | T826 | 117 |
| Ret | S696 | 79 |
| SPHK1 | Pan-specific | 107 |
| Src | Y529 | −56 |
| STAT3 | S727 | 50 |
| STK33 | Pan-specific | 58 |
| TRADD | Pan-specific | 77 |
| Tyrosine hydroxylase | S19 | −67 |
| ZAP70 | Pan-specific | 55 |
| Target protein name . | Phospho site (human) . | % CFC . |
|---|---|---|
| 4E-BP1 | S65 | −378 |
| Aik | Pan-specific | −55 |
| ALS2CR7[PFTAIRE2] | Pan-specific | −64 |
| Axl | Pan-specific | −81 |
| B23 (NPM) | T199 | 78 |
| CASP2 | Pan-specific | −55 |
| CREB1 | S129 + S133 | 85 |
| CREB1 | S133 | 56 |
| EGFR | Y1068 | 75 |
| eIF2Be | S540 | 57 |
| eIF4E | S209 | 93 |
| ErbB2[HER2] | Y1248 | 63 |
| Erk1/2 | T202 + Y204 | 83 |
| Erk5 | T218 + Y220 | 62 |
| FAK | S732 | 56 |
| Hsp25 | S86 | 53 |
| IGF1R | Pan-specific | −57 |
| Integrin a4 | S988 | 59 |
| IRAK4 | Pan-specific | −52 |
| JNK2 | Pan-specific | −82 |
| Jun | S73 | −83 |
| Kit | Y703 | 53 |
| MAPKAPK2 | T334 | 69 |
| Mcl-1 | Pan-specific | 675 |
| MEKK2[MAP3K2] | Pan-specific | −56 |
| MRLC2 | S18 | 66 |
| p16INK4 | Pan-specific | 77 |
| p38aMAPK | T180 + Y182 | 151 |
| PDGFRb | Y716 | 84 |
| PKA Ca/b | T197 | 70 |
| PKA R2a | S98 | −61 |
| PKBa (Akt1) | Pan-specific | 63 |
| PKBg (Akt3) | Pan-specific | −60 |
| PKCg | Pan-specific | 98 |
| PKCh | T655 | 51 |
| PKCq | S695 | 66 |
| PKCz | Pan-specific | 250 |
| PKR1 | Pan-specific | 51 |
| PKR1 | T451 | 66 |
| Plk1 | T210 | 73 |
| Plk2 | Pan-specific | 61 |
| PP1/Ca | T320 | 128 |
| PP2Cd | Pan-specific | 59 |
| PP4C | Pan-specific | 64 |
| PP5C | Pan-specific | 67 |
| PRK2[PKN2] | Pan-specific | 69 |
| PTEN | S370 | 121 |
| PTP1B | Pan-specific | 51 |
| Pyk2 | Pan-specific | 56 |
| Rac1/cdc42 | S71 | 52 |
| Rb | Pan-specific | 167 |
| Rb | S612 | 131 |
| Rb | S807 | 102 |
| Rb | T826 | 117 |
| Ret | S696 | 79 |
| SPHK1 | Pan-specific | 107 |
| Src | Y529 | −56 |
| STAT3 | S727 | 50 |
| STK33 | Pan-specific | 58 |
| TRADD | Pan-specific | 77 |
| Tyrosine hydroxylase | S19 | −67 |
| ZAP70 | Pan-specific | 55 |
Depicted is a list of differentially regulated proteins/phosphoproteins as detected in 32D_ITD627E cells. Shown are proteins with a more than 50% change in expression/phosphorylation as compared with 32D_ITD control cells (% CFC = % change from control).