Table 1

Induction of apoptosis in B-cell lymphoma lines

Cell lineOriginRelative expression of CD23*Apoptosis (%)
SKW6.4 Burkitt lymphoma 100 60 
SB Acute lymphoblastic leukemia 100 42 
DHL-4 Diffuse histiocytic lymphoma 
Daudi Burkitt lymphoma 
Raji Burkitt lymphoma 10 
Ramos Burkitt lymphoma 
Cell lineOriginRelative expression of CD23*Apoptosis (%)
SKW6.4 Burkitt lymphoma 100 60 
SB Acute lymphoblastic leukemia 100 42 
DHL-4 Diffuse histiocytic lymphoma 
Daudi Burkitt lymphoma 
Raji Burkitt lymphoma 10 
Ramos Burkitt lymphoma 
*

The expression of CD23 was evaluated by flow cytometry using phycoerythrin–anti-CD23 antibody. The relative fluorescence intensity (RFI) of antibody binding was determined by comparing the mean fluorescence intensity of phycoerythrin–anti-CD23 antibody binding to cells to that of the mean fluorescence intensity of phycoerythrin-labeled calibration beads (QuantiBrite, BD Biosciences), calculated as RFIsample/RFISKW6.4 cells × 100.

Percent apoptosis was measured by flow cytometric assessment of caspase-3 activation, as described in “Apoptosis assays.”

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