Comparison of the potency (IC50 values) of ABT-869 and other structurally unrelated FLT3 inhibitors for inhibiting the proliferation of MV4-11, MV4-11-R, MV4-11 + FLT3 ligand, and MV4-11-Survivin cells
| Drugs . | Structure . | IC50 (nM) . | |||
|---|---|---|---|---|---|
| MV4-11 . | MV4-11-R . | MV4-11 + FLT3 ligand* . | MV4-11-Survivin . | ||
| ABT-869 | 3-Aminoindazole | 6 | 52 | 40 | > 200 |
| FLT3 inhibitor III | 5-Phenyl-2-thiazolamine | 26 | 83 | 1300 | 713 |
| AG 1296 | Tyrphostin | 1657 | > 7000 | > 7000 | > 10 000 |
| SU5416 | 3-Substituted indolinone | 270 | 3039 | 3076 | > 10 000 |
| Drugs . | Structure . | IC50 (nM) . | |||
|---|---|---|---|---|---|
| MV4-11 . | MV4-11-R . | MV4-11 + FLT3 ligand* . | MV4-11-Survivin . | ||
| ABT-869 | 3-Aminoindazole | 6 | 52 | 40 | > 200 |
| FLT3 inhibitor III | 5-Phenyl-2-thiazolamine | 26 | 83 | 1300 | 713 |
| AG 1296 | Tyrphostin | 1657 | > 7000 | > 7000 | > 10 000 |
| SU5416 | 3-Substituted indolinone | 270 | 3039 | 3076 | > 10 000 |
Cells were seeded in 96-well culture plates at a density of 2 × 104 viable cells/100 μL/well in triplicates and were treated with each compound for 48 hours. Colorimetric MTS assay was used to determine the cytotoxicity. IC50 was determined by MTS assay and calculated with CalcuSyn software. Each experiment was performed in triplicate.
FLT3 ligand 50 ng/mL.