Multilineage potential of single CD34+lin−CD7− thymocytes
| Experiment no. . | No. of all clones/total cells plated (% plating efficiency) . | Clones with both lymphoid and myeloid potential (% of all clones) . |
|---|---|---|
| 1 | 37/384 (9.6) | 6 (16.2) |
| 2 | 13/84 (15.5) | 3 (23.0) |
| 3 | 42/192 (21.9) | 15 (35.7) |
| 4 | 18/96 (18.8) | 4 (22.2) |
| 5 | 32/96 (33.3) | 12 (37.5) |
| 6 | 15/96 (15.6) | 2 (13.3) |
| 7 | 41/192 (21.4) | 16 (39.0) |
| Mean ± SE | 19.4 ± 2.8 | 26.7 ± 4.0 |
| Experiment no. . | No. of all clones/total cells plated (% plating efficiency) . | Clones with both lymphoid and myeloid potential (% of all clones) . |
|---|---|---|
| 1 | 37/384 (9.6) | 6 (16.2) |
| 2 | 13/84 (15.5) | 3 (23.0) |
| 3 | 42/192 (21.9) | 15 (35.7) |
| 4 | 18/96 (18.8) | 4 (22.2) |
| 5 | 32/96 (33.3) | 12 (37.5) |
| 6 | 15/96 (15.6) | 2 (13.3) |
| 7 | 41/192 (21.4) | 16 (39.0) |
| Mean ± SE | 19.4 ± 2.8 | 26.7 ± 4.0 |
Data from 7 independent experiments analyzing individual CD34+lin−CD7− cells cocultured on OP9 stroma. Lympho-myeloid clones were recorded if markers of both lymphoid (ie, B and/or NK and/or T cell) and myeloid (ie, myeloid and/or erythroid) differentiation were identified by either FACS and/or RT-PCR.