Table 1. Genetic features and the... | American Society of Hematology

Table 1.

Genetic features and the individual response of the parameters investigated within each CLL sample to establish the miRNA106b-initiated signaling cascade, after exposure to LBH589 or MS275

Patient no.	Percentage of cells with del17p (p53)*	AcH3	E2F1	MCM7	miRNA 106b	Itch protein	p73 protein	PUMA mRNA	PUMA protein	Cleaved caspase-9	Annexin positivity	ZAP70	p73 mRNA
1	0	↑			—†	↓	↑	↑	↑	↑	↑	positive	↓
2	0	↑			—	↓	↑	—	↑	↑	↑	—	—
3	0	↑			—	↓	↑	—	↑	↑	↑	positive	—
4	—‖	↑			—	↓	↑	↑	↑	↑	↑	—	↓
5	69%	↑			—	↓	↑	—	↑	↑	↑	positive	—
6	12 double deletion, 9 monosomy‖	↑			—	↓	↑	↑	↑	↑	↑	positive	↑
7	0	↑	neg	∼	∼	↓	↑	—	↑	↑	↑	—	—
8	36	↑	+	↑	↑	↓	↑	—	not detectable	↑	↑	—	—
9	89	↑	+	↑	↑	↓	↑	—	↑	↑	↑	positive	—
10	0	↑			—	↓	∼‡	∼‡	not detectable	partial	< 20%§	negative	↓
11	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↓
12	18	↑			—	↓	↑	↓	↑	↑	↑	—	↑
13	0	↑			—	↓	∼‡	↑	↑	↑	↑	—	↓
14	0	↑			—	↓	↑	—	↑	↑	↑	positive	—
15	0	↑			—	↓	↑	↑	∼	↑	↑	negative	↓
16	67.5	↑			—	↓	↑	↑	↑	↑	↑	—	↑
17	0	↑			—	↓	↑	∼	↑	↑	↑	—	↓
18	0	↑			—	↓	↑	↑	↑	↑	↑	positive	∼
19	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↑
20	0	↑			—	↓	∼	↓	↑	↑	↑	—	↓
21	86	↑	+	↑	↑	↓	∼	↓	↓	↑	< 20%	positive	↓
22	9.5	↑	+	↑	↑	↓	↑	↑	↑	↑	↑	positive	↓
23	70	↑			—	↓	↑	↓	↓	↑	↑	—	↑
24	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↓
25	82	↑	neg	∼	∼	↓	↑	↓	∼	↑	↑	—	↓
26	93	↑	+	↑	↑	↓	↑	—	↑	↑	↑	positive	—
27	0	↑	+	∼	∼	↓	↑	—	↑	↑	↑	negative	—
28	0	↑	+	↑	↑	↓	↑	—	↑	↑	↑	negative	—
29	0	↑	neg	∼	∼	↓	∼	—	↑	∼	< 20%	positive	—
30	0	—			—	—	—	—	—	—	—	positive	—
31	0	↑			—	↓	↑	↑	∼	↑	↑	—	↑
32	0	↑	+	↑	↑	↓	↑	↑	∼	↑	↑	positive	↓
33	—	↑	+	↑	↑	↓	↑	↑	↑	↑	↑	—	—
34	18	—			—	—	—	↑	—	—	—	positive	—
35	0	↑	+	∼	↑	↓	↑	—	↑	↑	↑	positive	—
36	0	↑	neg	∼	∼	↓	↑	—	↑	↑	↑	positive	—
37¶	0	↑	—	—	∼	∼	∼	—	—	partial	< 20%	positive	—
38¶	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
39**	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
40	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	negative	—
41	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
42	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	—	—
43	67	↑	+	↑	↑							positive
44	—		neg	∼	∼							—
46	—	↑	+	∼	∼							—
47	—	↑	+	↑	↑							—

Patient no.	Percentage of cells with del17p (p53)*	AcH3	E2F1	MCM7	miRNA 106b	Itch protein	p73 protein	PUMA mRNA	PUMA protein	Cleaved caspase-9	Annexin positivity	ZAP70	p73 mRNA
1	0	↑			—†	↓	↑	↑	↑	↑	↑	positive	↓
2	0	↑			—	↓	↑	—	↑	↑	↑	—	—
3	0	↑			—	↓	↑	—	↑	↑	↑	positive	—
4	—‖	↑			—	↓	↑	↑	↑	↑	↑	—	↓
5	69%	↑			—	↓	↑	—	↑	↑	↑	positive	—
6	12 double deletion, 9 monosomy‖	↑			—	↓	↑	↑	↑	↑	↑	positive	↑
7	0	↑	neg	∼	∼	↓	↑	—	↑	↑	↑	—	—
8	36	↑	+	↑	↑	↓	↑	—	not detectable	↑	↑	—	—
9	89	↑	+	↑	↑	↓	↑	—	↑	↑	↑	positive	—
10	0	↑			—	↓	∼‡	∼‡	not detectable	partial	< 20%§	negative	↓
11	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↓
12	18	↑			—	↓	↑	↓	↑	↑	↑	—	↑
13	0	↑			—	↓	∼‡	↑	↑	↑	↑	—	↓
14	0	↑			—	↓	↑	—	↑	↑	↑	positive	—
15	0	↑			—	↓	↑	↑	∼	↑	↑	negative	↓
16	67.5	↑			—	↓	↑	↑	↑	↑	↑	—	↑
17	0	↑			—	↓	↑	∼	↑	↑	↑	—	↓
18	0	↑			—	↓	↑	↑	↑	↑	↑	positive	∼
19	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↑
20	0	↑			—	↓	∼	↓	↑	↑	↑	—	↓
21	86	↑	+	↑	↑	↓	∼	↓	↓	↑	< 20%	positive	↓
22	9.5	↑	+	↑	↑	↓	↑	↑	↑	↑	↑	positive	↓
23	70	↑			—	↓	↑	↓	↓	↑	↑	—	↑
24	0	↑			—	↓	↑	↑	↑	↑	↑	positive	↓
25	82	↑	neg	∼	∼	↓	↑	↓	∼	↑	↑	—	↓
26	93	↑	+	↑	↑	↓	↑	—	↑	↑	↑	positive	—
27	0	↑	+	∼	∼	↓	↑	—	↑	↑	↑	negative	—
28	0	↑	+	↑	↑	↓	↑	—	↑	↑	↑	negative	—
29	0	↑	neg	∼	∼	↓	∼	—	↑	∼	< 20%	positive	—
30	0	—			—	—	—	—	—	—	—	positive	—
31	0	↑			—	↓	↑	↑	∼	↑	↑	—	↑
32	0	↑	+	↑	↑	↓	↑	↑	∼	↑	↑	positive	↓
33	—	↑	+	↑	↑	↓	↑	↑	↑	↑	↑	—	—
34	18	—			—	—	—	↑	—	—	—	positive	—
35	0	↑	+	∼	↑	↓	↑	—	↑	↑	↑	positive	—
36	0	↑	neg	∼	∼	↓	↑	—	↑	↑	↑	positive	—
37¶	0	↑	—	—	∼	∼	∼	—	—	partial	< 20%	positive	—
38¶	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
39**	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
40	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	negative	—
41	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	positive	—
42	0	↑	+	↑	↑	↓	↑	—	—	↑	↑	—	—
43	67	↑	+	↑	↑							positive
44	—		neg	∼	∼							—
46	—	↑	+	∼	∼							—
47	—	↑	+	↑	↑							—

neg indicates undetectable protein levels.

CLL samples were classified according their p53 FISH status.

†

No sample.

‡

No change from control.

A total of 20% was used as a cutoff point for determining annexin positivity.

‖

Negative in p21 functional assay.

CLL sample exposed to 3 μM SAHA.

CLL sample exposed to 3 μM MS275.

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