CpG ODNs promote rejection by ligation of donor BM TLR9 but not host TLR9
| Donor . | Recipient . | CpG . | Survival . | Engraftment rate . | Donor chimerism, mean % ± 1 SEM . |
|---|---|---|---|---|---|
| BALB/c | B6 | No | 15/18 | 14/15 | 72 ± 6 |
| BALB/c | B6 | Yes | 10/18 | 6/10* | 34 ± 11* |
| BALB/c TLR9−/− | B6 | No | 28/28 | 26/28 | 65 ± 6 |
| BALB/c TLR9−/− | B6 | Yes | 25/28 | 23/25 | 64 ± 6 |
| BALB/c | B6 TLR9−/− | No | 28/28 | 21/28 | 58 ± 8 |
| BALB/c | B6 TLR9−/− | Yes | 20/32* | 6/20* | 19 ± 8* |
| Donor . | Recipient . | CpG . | Survival . | Engraftment rate . | Donor chimerism, mean % ± 1 SEM . |
|---|---|---|---|---|---|
| BALB/c | B6 | No | 15/18 | 14/15 | 72 ± 6 |
| BALB/c | B6 | Yes | 10/18 | 6/10* | 34 ± 11* |
| BALB/c TLR9−/− | B6 | No | 28/28 | 26/28 | 65 ± 6 |
| BALB/c TLR9−/− | B6 | Yes | 25/28 | 23/25 | 64 ± 6 |
| BALB/c | B6 TLR9−/− | No | 28/28 | 21/28 | 58 ± 8 |
| BALB/c | B6 TLR9−/− | Yes | 20/32* | 6/20* | 19 ± 8* |
Recipient mice were irradiated (6.0 Gy TBI) on d−1 and infused with 10 × 106 TCD donor BM cells from indicated strain on d0. CpG ODNs were given intraperitoneally on d0 and d7 (100 μg/dose). Survival indicates proportion of mice that received donor BM that survived to PBL phenotyping at approximately d35 after BMT. Engraftment rate indicates proportion of phenotyped mice that had a minimum of 5% donor chimerism.
P < .05 compared with relevant untreated control.