Reactions of DDAbs 1-16 with wild-type (construct 1), truncated (construct 3), and mutant (constructs 4-6) GPIIIa
| DDAb . | Construct 1 . | Construct 3 . | Construct 4 A50S, R62Q, D66A . | Construct 5 E52, 55A . | Construct 6 S53A . | Blocked by AP3* . |
|---|---|---|---|---|---|---|
| 1 | + | + | − | + | + | + |
| 2 | + | + | − | + | + | + |
| 3 | + | + | − | + | + | + |
| 4 | + | + | − | + | + | + |
| 5 | + | + | − | + | + | + |
| 6 | + | + | − | + | + | + |
| 7 | + | + | − | + | + | + |
| 8 | + | + | − | − | − | + |
| 9 | + | + | − | − | − | + |
| 10 | + | + | + | − | + | + |
| 11 | + | + | + | − | + | + |
| 12 | + | + | − | − | + | − |
| 13 | + | + | − | + | + | − |
| 14 | + | + | + | − | + | − |
| 15 | + | − | − | − | + | − |
| 16 | + | − | + | + | + | − |
| DDAb . | Construct 1 . | Construct 3 . | Construct 4 A50S, R62Q, D66A . | Construct 5 E52, 55A . | Construct 6 S53A . | Blocked by AP3* . |
|---|---|---|---|---|---|---|
| 1 | + | + | − | + | + | + |
| 2 | + | + | − | + | + | + |
| 3 | + | + | − | + | + | + |
| 4 | + | + | − | + | + | + |
| 5 | + | + | − | + | + | + |
| 6 | + | + | − | + | + | + |
| 7 | + | + | − | + | + | + |
| 8 | + | + | − | − | − | + |
| 9 | + | + | − | − | − | + |
| 10 | + | + | + | − | + | + |
| 11 | + | + | + | − | + | + |
| 12 | + | + | − | − | + | − |
| 13 | + | + | − | + | + | − |
| 14 | + | + | + | − | + | − |
| 15 | + | − | − | − | + | − |
| 16 | + | − | + | + | + | − |
Reactions shown were obtained in the presence of quinine. No reactions were obtained in the absence of quinine.
+ indicates positive reaction; and −, no reaction.
Reactions with platelet GPIIIa blocked by monoclonal AP3.