Oligonucleotide primers used in this study
| Gene/fragment location . | Method . | Forward sequence . | Reverse sequence . |
|---|---|---|---|
| ET-BR promoter (−1259/+493) | PCR | aatcatggtaccacatggtgcgtgataacttg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−867/+493) | PCR | tagtcgtggtacctgtctgtattcctcccgttac | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−555/+493) | PCR | tagtcgtggtaccgtctccattcgtcgctctg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−392/+493) | PCR | tagtcgtggtaccgtctccattcgtcgctctg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−392/+493)-HIF-1α-M1 | SDM | cttgagttacttttgagcagggatactggcgaagaggctg | cagcctcttcgccagtatccctgctcaaaagtaactcaag |
| ET-BR promoter (−392/+493)-HIF-1α-M2 | SDM | ctgtattcctcccgttacaggaaagagttcggagctttg | caaagctccgaactctttcctgtaacgggaggaatacag |
| HIF-1α consensus oligo | EMSA | tctgtacgtgaccacactcacctc | gaggtgagtgtggtcacgtacaga |
| ET-1 promoter (−606/+5) | ChIP | cctcacggatctttctccgat | gtctgacttggacagctctct |
| ET-BR promoter (−619/+2) | ChIP | tgagaagtctctgcgggtttc | atgctgctacctgctccagaa |
| Gene/fragment location . | Method . | Forward sequence . | Reverse sequence . |
|---|---|---|---|
| ET-BR promoter (−1259/+493) | PCR | aatcatggtaccacatggtgcgtgataacttg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−867/+493) | PCR | tagtcgtggtacctgtctgtattcctcccgttac | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−555/+493) | PCR | tagtcgtggtaccgtctccattcgtcgctctg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−392/+493) | PCR | tagtcgtggtaccgtctccattcgtcgctctg | acgtacagatctcgccctttaccttgtaga |
| ET-BR promoter (−392/+493)-HIF-1α-M1 | SDM | cttgagttacttttgagcagggatactggcgaagaggctg | cagcctcttcgccagtatccctgctcaaaagtaactcaag |
| ET-BR promoter (−392/+493)-HIF-1α-M2 | SDM | ctgtattcctcccgttacaggaaagagttcggagctttg | caaagctccgaactctttcctgtaacgggaggaatacag |
| HIF-1α consensus oligo | EMSA | tctgtacgtgaccacactcacctc | gaggtgagtgtggtcacgtacaga |
| ET-1 promoter (−606/+5) | ChIP | cctcacggatctttctccgat | gtctgacttggacagctctct |
| ET-BR promoter (−619/+2) | ChIP | tgagaagtctctgcgggtttc | atgctgctacctgctccagaa |
Bold and underlined sequences highlight the specific mutations.
ChIP indicates chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; PCR, polymerase chain reaction; and SDM, site-directed mutagenesis.