Table 1

Primers for vector construction

PrimerStrandSequenceRE site*Constructs
PZ-F Sense 5′-TCCGGATCCGAATGGCAGGCTGCGTCCCAC-3′ BamHI Full-length PZ and ZPro 
PZ-R Antisense 5′-GCTGGGATCCAGTTAGTTCATGATCTGTTTAAA-3′ BamHI Full-length PZ and PZC 
FX-F Sense 5′-CTGGGATCCCACCATGGGGCGCCCACTGCAC-3′ BamHI Full-length FX and XPro 
FX-R1 Antisense 5′-GTGGGATCCTCACTTTAATGGAGAGGACGTTAT-3′ BamHI Full-length FX and FXC 
FX-R2 Antisense 5′-TCACTCCATGGGAGAGGACGTTATGACCTC-3′ NcoI FXMetLuc 
MLUC-F1 Sense 5′-CGCCATCATGAACATCAAGG-3′ BspHI PZMetLuc(+Sig) 
MLUC-F2 Sense 5′-TGGTGCTCATGAAGAGCACCGAGTTCGA-3′ BspHI PZMetLuc, FXMetLuc 
MLUC-R Antisense 5′-TGGCTGATTATGATCTAGAG-3′ XbaI PZMetLuc, FXMetLuc 
XPPZGla-F Sense 5′-GGTCACGAGGGCGGGCTCCTATCTTCTGGA-3′  XPPZGla 
XPPZGla-R Antisense 5′-AGGAGCCCGCCCTCGTGACCCTCGCCAGGA-3′  XPPZGla 
ZPPXGla-F Sense 5′-GTGGAAGCGTGCCAATTCCTTTCTTGAAGA-3′  ZPPXGla 
ZPPXGla-R Antisense 5′-AGGAATTGGCACGCTTCCACCTCACCAGAA-3′  ZPPXGla 
ZPPmut-F Sense 5′-CTGGTGGGGTGGAAGGGTGCGG-3′  PZPPmut 
ZPPmut-R Antisense 5′-CCGCACCCTTCCACCCCACCAG-3′  PZPPmut 
ZGla-R Antisense 5′-GCACGCCATGGCCTTATATCGTCTCC-3′ NcoI ZPPZGlaMetLuc, XPPZGlaMetLuc 
XGla-R Antisense 5′-CTGGTCCATGGCTTTGTATTTATTCCA-3′ NcoI ZPPXGlaMetLuc, XPPXGlaMetLuc 
PrimerStrandSequenceRE site*Constructs
PZ-F Sense 5′-TCCGGATCCGAATGGCAGGCTGCGTCCCAC-3′ BamHI Full-length PZ and ZPro 
PZ-R Antisense 5′-GCTGGGATCCAGTTAGTTCATGATCTGTTTAAA-3′ BamHI Full-length PZ and PZC 
FX-F Sense 5′-CTGGGATCCCACCATGGGGCGCCCACTGCAC-3′ BamHI Full-length FX and XPro 
FX-R1 Antisense 5′-GTGGGATCCTCACTTTAATGGAGAGGACGTTAT-3′ BamHI Full-length FX and FXC 
FX-R2 Antisense 5′-TCACTCCATGGGAGAGGACGTTATGACCTC-3′ NcoI FXMetLuc 
MLUC-F1 Sense 5′-CGCCATCATGAACATCAAGG-3′ BspHI PZMetLuc(+Sig) 
MLUC-F2 Sense 5′-TGGTGCTCATGAAGAGCACCGAGTTCGA-3′ BspHI PZMetLuc, FXMetLuc 
MLUC-R Antisense 5′-TGGCTGATTATGATCTAGAG-3′ XbaI PZMetLuc, FXMetLuc 
XPPZGla-F Sense 5′-GGTCACGAGGGCGGGCTCCTATCTTCTGGA-3′  XPPZGla 
XPPZGla-R Antisense 5′-AGGAGCCCGCCCTCGTGACCCTCGCCAGGA-3′  XPPZGla 
ZPPXGla-F Sense 5′-GTGGAAGCGTGCCAATTCCTTTCTTGAAGA-3′  ZPPXGla 
ZPPXGla-R Antisense 5′-AGGAATTGGCACGCTTCCACCTCACCAGAA-3′  ZPPXGla 
ZPPmut-F Sense 5′-CTGGTGGGGTGGAAGGGTGCGG-3′  PZPPmut 
ZPPmut-R Antisense 5′-CCGCACCCTTCCACCCCACCAG-3′  PZPPmut 
ZGla-R Antisense 5′-GCACGCCATGGCCTTATATCGTCTCC-3′ NcoI ZPPZGlaMetLuc, XPPZGlaMetLuc 
XGla-R Antisense 5′-CTGGTCCATGGCTTTGTATTTATTCCA-3′ NcoI ZPPXGlaMetLuc, XPPXGlaMetLuc 
*

Restriction enzyme (RE) site indicated italics in primer sequence.

View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal