Evaluation of the coefficient of variation in the spot intensity in the different T-cell subsets
.
N
.
CM
.
EM
.
E
.
CV mean (%)
10.6
10.3
11.4
11.0
.
N
.
CM
.
EM
.
E
.
CV mean (%)
10.6
10.3
11.4
11.0
A total of 200 triplicate spots and 15 different signaling components were examined for each T-cell subset. The means of the coefficient of variation in signal ratio to β-actin (CV = CVprotein + CVβ-actin) within triplicate spots were calculated from 10 subjects.