Table 1 Target ligand expression on... | American Society of Hematology

Table 1

Target ligand expression on HUVECs by flow cytometry

Antibody (target)	% positive HUVECs cultured on plastic (MFI)*	% positive HUVECs cultured on gelatin (MFI)*
P3 (negative control)	2.3 ± 0.6 (113 ± 6.1)	2 ± 0.6 (129 ± 8.3)
Anti-αvβ3	49.7 ± 3.5 (127 ± 4.4)	50 ± 2.5 (304 ± 19.8)
Anti-CD44	6 ± 1.5 (121 ± 3.6)	9 ± 3.5 (131 ± 8.2)
Antithrombospondin†	56.3 ± 3.5 (159 ± 8.1)	87 ± 2.5 (800 ± 23)
Antifibronectin†	8.3 ± 1.5 (170 ± 4.6)	59 ± 4 (693 ± 21.8)
Anti–E-selectin†	2.3 ± 0.6 (115 ± 7)	37 ± 2.6 (252 ± 4.7)
Anti–P-selectin	1.7 ± 0.6 (108 ± 2.5)	3 ± 0.6 (174 ± 3.6)

Antibody (target)	% positive HUVECs cultured on plastic (MFI)*	% positive HUVECs cultured on gelatin (MFI)*
P3 (negative control)	2.3 ± 0.6 (113 ± 6.1)	2 ± 0.6 (129 ± 8.3)
Anti-αvβ3	49.7 ± 3.5 (127 ± 4.4)	50 ± 2.5 (304 ± 19.8)
Anti-CD44	6 ± 1.5 (121 ± 3.6)	9 ± 3.5 (131 ± 8.2)
Antithrombospondin†	56.3 ± 3.5 (159 ± 8.1)	87 ± 2.5 (800 ± 23)
Antifibronectin†	8.3 ± 1.5 (170 ± 4.6)	59 ± 4 (693 ± 21.8)
Anti–E-selectin†	2.3 ± 0.6 (115 ± 7)	37 ± 2.6 (252 ± 4.7)
Anti–P-selectin	1.7 ± 0.6 (108 ± 2.5)	3 ± 0.6 (174 ± 3.6)

Mean percentage and mean of mean fluorescence intensity (MFI) of positive cells are shown (n = 3 different experiments, ± SEM).

†

P < .001 for % HUVECs that were positive with indicated antibody when cells were cultured on gelatin, compared with % HUVECs positive when cells were cultured on plastic.

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