Sequence of primers and probes used for polymorphism genotyping
| Gene . | Primers and probes . | ||
|---|---|---|---|
| Name . | Reagent . | Sequence . | |
| CYP3A4 | CYP3A4*1B-G | MGB probe, FAM dye-labeled | 5′-ACAAGGGCAGGAGAG |
| CYP3A4*1B-A | MGB probe, VIC dye-labeled | 5′-CAAGGGCAAGAGAG | |
| CYP3A4F | PCR primer | 5′-CAGGCATAGGTAAAGATCTGTAGGTG | |
| CYP3A4R | PCR primer | 5′-CAGAAACTCAAGTGGAGCCATTG | |
| UGT1A1 | UGT1A1F | PCR primer; FAM dye-labeled | 5′-TACAGTCACGTGACACAGTCAAAC |
| UGT1A1R | PCR primer | 5′-GCCTTTGCTCCTGCCAGAGGTTCG | |
| GSTM1 | GSTM1F | PCR primer; FAM dye-labeled | 5′-tail*-CGCCATCTTGTGCTACATTG* |
| GSTM1R | PCR primer | 5′-tail-GCCCCAAATCCAAACTCTG* | |
| GSTT1 | GSTT1F | PCR primer; FAM dye-labeled | 5′-tail-TCCCCAGTCTGTACCCTTTTC* |
| GSTT1R | PCR primer | 5′-tail-TGGCCTTCAGAATGACCTCA* | |
| GAPDH | GAPDHF | PCR primer; FAM dye-labeled | 5′-tail-TTGCCTCTTGTCTCTTAGAT* |
| GAPDHR | PCR primer | 5′-tail-GGCAACAATATCCACTTTAC* | |
| Gene . | Primers and probes . | ||
|---|---|---|---|
| Name . | Reagent . | Sequence . | |
| CYP3A4 | CYP3A4*1B-G | MGB probe, FAM dye-labeled | 5′-ACAAGGGCAGGAGAG |
| CYP3A4*1B-A | MGB probe, VIC dye-labeled | 5′-CAAGGGCAAGAGAG | |
| CYP3A4F | PCR primer | 5′-CAGGCATAGGTAAAGATCTGTAGGTG | |
| CYP3A4R | PCR primer | 5′-CAGAAACTCAAGTGGAGCCATTG | |
| UGT1A1 | UGT1A1F | PCR primer; FAM dye-labeled | 5′-TACAGTCACGTGACACAGTCAAAC |
| UGT1A1R | PCR primer | 5′-GCCTTTGCTCCTGCCAGAGGTTCG | |
| GSTM1 | GSTM1F | PCR primer; FAM dye-labeled | 5′-tail*-CGCCATCTTGTGCTACATTG* |
| GSTM1R | PCR primer | 5′-tail-GCCCCAAATCCAAACTCTG* | |
| GSTT1 | GSTT1F | PCR primer; FAM dye-labeled | 5′-tail-TCCCCAGTCTGTACCCTTTTC* |
| GSTT1R | PCR primer | 5′-tail-TGGCCTTCAGAATGACCTCA* | |
| GAPDH | GAPDHF | PCR primer; FAM dye-labeled | 5′-tail-TTGCCTCTTGTCTCTTAGAT* |
| GAPDHR | PCR primer | 5′-tail-GGCAACAATATCCACTTTAC* | |
Primers and probes were chosen using Oligo version 6.69 (Molecular Biology Insights, Cascade, CO) and Primer Express (Applied Biosystems).
Tail: additional sequences designed to improve Quantitative Multiplex PCR of Short Fluorescent Fragment, provided under confidential agreement by Inserm U614 (Dr Mario Tosi, Rouen, France).