Hybridization data of specific probe sets for 6 candidate stage-specific exons
| Gene (exon length) . | Probe set location . | Probe set ID . | Splicing index magnitude . | P . | Direction . | Comments . |
|---|---|---|---|---|---|---|
| EPB4163nt | Alternative exon | 217437 | 2.16 | 9.0E-03* | Up | In-frame insertion of 21 amino acids |
| 5′ junction | 504900 | 2.20 | 7.5E-07 | Up | ||
| 3′ junction | 224956 | 2.33 | 1.6E-09 | Up | ||
| Skip junction | 366937 | −0.93 | 1.2E-04 | Down | ||
| ARFIP196nt | Alternative exon | 497178 | 0.92 | 1.6E-05 | Up | In-frame insertion of 32 amino acids |
| 5′ junction | 425212 | 0.82 | 1.8E-04 | Up | ||
| 3′ junction | 79253 | 0.97 | 3.8E-03* | Up | ||
| Skip junction | 524645 | −0.62 | 2.4E-05 | Down | ||
| ATP11C66nt | Alternative exon | 462451 | 0.67 | 7.2E-02* | Up | Insertion of stop codon creating alternative C-terminal sequence and possible NMD |
| Alternative exon | 20030 | 0.63 | 8.3E-03* | Up | ||
| 5′ junction | 521875 | 0.86 | 5.8E-04 | Up | ||
| 3′ junction | 645995 | 1.01 | 1.8E-05 | Up | ||
| Skip junction | 603987 | −1.03 | 3E-04 | Down | ||
| HNRPLL83nt | Alternative exon | 376564 | 1.11 | 3.2E-04 | Up | Insertion of stop codon creating alternative C-terminal sequence and possible NMD |
| Alternative exon | 418887 | 1.32 | 7.0E-04 | Up | ||
| 5′ junction | 291050 | 1.39 | 3.6E-05 | Up | ||
| 3′ junction | 679040 | 1.24 | 1.0E-02* | Up | ||
| Skip junction | 376125 | −0.50 | 3.6E-04 | Down | ||
| MBNL295nt | Alternative exon | 253023 | 0.69 | 5.6E-05 | Up | Creates alternative C-terminal sequence; NMD doubtful because of 3′ location of insertion |
| 5′ junction | 473479 | 1.01 | 1.3E-06 | Up | ||
| 3′ junction | 636764 | 0.94 | 3.8E-04 | Up | ||
| Skip junction | 276844 | −2.55 | 1.7E-05 | Down | ||
| PLD1114nt | Alternative exon | 644361 | 0.52 | 7.7E-05 | Up | In-frame insertion of 38 amino acids |
| 5′ junction | 657499 | 0.57 | 6.3E-05 | Up | ||
| 3′ junction | 652587 | 0.56 | 7.3E-02* | Up | ||
| Skip junction | 421462 | −1.87 | 1.2E-04 | Down | ||
| SNRP7072nt | Alternative exon | 178662 | 1.14 | 7.0E-03* | Up | Insertion of stop codon creates alternative C-terminal sequence and possible NMD |
| 5′ junction | 38132 | 1.74 | 2.7E-06 | Up | ||
| 3′ junction | 475296 | 0.68 | 1.6E-03 | Up | ||
| Skip junction | 585925 | −1.08 | 1.2E-04 | Down | ||
| Gene (exon length) . | Probe set location . | Probe set ID . | Splicing index magnitude . | P . | Direction . | Comments . |
|---|---|---|---|---|---|---|
| EPB4163nt | Alternative exon | 217437 | 2.16 | 9.0E-03* | Up | In-frame insertion of 21 amino acids |
| 5′ junction | 504900 | 2.20 | 7.5E-07 | Up | ||
| 3′ junction | 224956 | 2.33 | 1.6E-09 | Up | ||
| Skip junction | 366937 | −0.93 | 1.2E-04 | Down | ||
| ARFIP196nt | Alternative exon | 497178 | 0.92 | 1.6E-05 | Up | In-frame insertion of 32 amino acids |
| 5′ junction | 425212 | 0.82 | 1.8E-04 | Up | ||
| 3′ junction | 79253 | 0.97 | 3.8E-03* | Up | ||
| Skip junction | 524645 | −0.62 | 2.4E-05 | Down | ||
| ATP11C66nt | Alternative exon | 462451 | 0.67 | 7.2E-02* | Up | Insertion of stop codon creating alternative C-terminal sequence and possible NMD |
| Alternative exon | 20030 | 0.63 | 8.3E-03* | Up | ||
| 5′ junction | 521875 | 0.86 | 5.8E-04 | Up | ||
| 3′ junction | 645995 | 1.01 | 1.8E-05 | Up | ||
| Skip junction | 603987 | −1.03 | 3E-04 | Down | ||
| HNRPLL83nt | Alternative exon | 376564 | 1.11 | 3.2E-04 | Up | Insertion of stop codon creating alternative C-terminal sequence and possible NMD |
| Alternative exon | 418887 | 1.32 | 7.0E-04 | Up | ||
| 5′ junction | 291050 | 1.39 | 3.6E-05 | Up | ||
| 3′ junction | 679040 | 1.24 | 1.0E-02* | Up | ||
| Skip junction | 376125 | −0.50 | 3.6E-04 | Down | ||
| MBNL295nt | Alternative exon | 253023 | 0.69 | 5.6E-05 | Up | Creates alternative C-terminal sequence; NMD doubtful because of 3′ location of insertion |
| 5′ junction | 473479 | 1.01 | 1.3E-06 | Up | ||
| 3′ junction | 636764 | 0.94 | 3.8E-04 | Up | ||
| Skip junction | 276844 | −2.55 | 1.7E-05 | Down | ||
| PLD1114nt | Alternative exon | 644361 | 0.52 | 7.7E-05 | Up | In-frame insertion of 38 amino acids |
| 5′ junction | 657499 | 0.57 | 6.3E-05 | Up | ||
| 3′ junction | 652587 | 0.56 | 7.3E-02* | Up | ||
| Skip junction | 421462 | −1.87 | 1.2E-04 | Down | ||
| SNRP7072nt | Alternative exon | 178662 | 1.14 | 7.0E-03* | Up | Insertion of stop codon creates alternative C-terminal sequence and possible NMD |
| 5′ junction | 38132 | 1.74 | 2.7E-06 | Up | ||
| 3′ junction | 475296 | 0.68 | 1.6E-03 | Up | ||
| Skip junction | 585925 | −1.08 | 1.2E-04 | Down | ||
NMD indicates nonsense mediated decay.
Probe set did not pass significance filters (P < .001).