Comparison of commercially available D-dimer assays
| Technique . | Commercial kits . | Sample type . | Sensitivity . | Specificity . | Automation . | Series . |
|---|---|---|---|---|---|---|
| Microplate ELISA* | Asserachrom DDI (Stago); Enzygnost (Dade-Behring)* | Plasma | High | Low | Manual | Indik et al,109 Bournameaux et al110 |
| ELISA and fluorescence (ELFA) | Vidas DD (bioMérieux); AxSym D-dimer (Abbott); Stratus D-dimer (Dade-Behring) | Plasma | High | Low | Automated | Mountain et al,28 van Belle et al60 |
| ELISA and chemiluminescence | Immulite (Siemens); Pathfast (Mitsubishi) | Plasma | High | Low | Automated | Fukuda et al,111 Dempfle et al112 |
| Immunofiltration and sandwich-type | NycoCard (Nycomed); Cardiac D-dimer (Roche) | Plasma | High High | Low-intermediate High | Automated | Scarano et al,35 Killick et al113 |
| Semi-quantitative latex agglutination | Dimertest latex (IL); Fibrinosticon (bioMérieux); DDI latex (Stago) | Plasma | Intermediate | Intermediate | Manual | Veitl et al,114 Sukhu et al115 |
| Manual whole-blood agglutination | SimpliRED (Agen); Clearview Simplify D-dimer (Agen) | Whole blood | High- intermediate | Intermediate | Manual | de Groot et al,39 Toulon et al116 |
| Second-generation latex agglutination (immunoturbidimetric) | TinaQuant (Roche); Liatest (Stago); Automated Dimertest (Agen); MDA D-dimer (bioMérieux); Turbiquant (Dade-Behring) | Plasma | High | Intermediate | Automated | Froehling et al,30 Curtin et al37 |
| Technique . | Commercial kits . | Sample type . | Sensitivity . | Specificity . | Automation . | Series . |
|---|---|---|---|---|---|---|
| Microplate ELISA* | Asserachrom DDI (Stago); Enzygnost (Dade-Behring)* | Plasma | High | Low | Manual | Indik et al,109 Bournameaux et al110 |
| ELISA and fluorescence (ELFA) | Vidas DD (bioMérieux); AxSym D-dimer (Abbott); Stratus D-dimer (Dade-Behring) | Plasma | High | Low | Automated | Mountain et al,28 van Belle et al60 |
| ELISA and chemiluminescence | Immulite (Siemens); Pathfast (Mitsubishi) | Plasma | High | Low | Automated | Fukuda et al,111 Dempfle et al112 |
| Immunofiltration and sandwich-type | NycoCard (Nycomed); Cardiac D-dimer (Roche) | Plasma | High High | Low-intermediate High | Automated | Scarano et al,35 Killick et al113 |
| Semi-quantitative latex agglutination | Dimertest latex (IL); Fibrinosticon (bioMérieux); DDI latex (Stago) | Plasma | Intermediate | Intermediate | Manual | Veitl et al,114 Sukhu et al115 |
| Manual whole-blood agglutination | SimpliRED (Agen); Clearview Simplify D-dimer (Agen) | Whole blood | High- intermediate | Intermediate | Manual | de Groot et al,39 Toulon et al116 |
| Second-generation latex agglutination (immunoturbidimetric) | TinaQuant (Roche); Liatest (Stago); Automated Dimertest (Agen); MDA D-dimer (bioMérieux); Turbiquant (Dade-Behring) | Plasma | High | Intermediate | Automated | Froehling et al,30 Curtin et al37 |
Adapted from Righini et al117 with permission.
ELISA indicates enzyme-linked immunosorbent assay.
This assay can only be performed in batches and has a relatively longer turnaround time.