Frequency and range of mutations in a 500-bp–long region in the intron downstream of the rearranged VHDHJH4 joints of splenic GC B cells of 2 independent experiments
| Genotype . | No. clones . | No. mutated clones . | Range, mutations/clone . | No. mutations/no. bp (%) . |
|---|---|---|---|---|
| CD40/LMP1+//CD40−/− | 44 | 38 | 1-11 | 130/22000 (0.59) |
| CD40+/+ | 43 | 37 | 1-11 | 146/21500 (0.68) |
| CD40/LMP1+//CD40−/− | 41 | 35 | 1-6 | 97/20500 (0.47) |
| CD40+/+ | 41 | 29 | 1-6 | 74/20500 (0.36) |
| Genotype . | No. clones . | No. mutated clones . | Range, mutations/clone . | No. mutations/no. bp (%) . |
|---|---|---|---|---|
| CD40/LMP1+//CD40−/− | 44 | 38 | 1-11 | 130/22000 (0.59) |
| CD40+/+ | 43 | 37 | 1-11 | 146/21500 (0.68) |
| CD40/LMP1+//CD40−/− | 41 | 35 | 1-6 | 97/20500 (0.47) |
| CD40+/+ | 41 | 29 | 1-6 | 74/20500 (0.36) |
Splenic GC and naive B cells were isolated 14 days after immunization with NP-CGG. PCR was performed from 40 000 cell equivalents with primers annealing in the framework 3 region of most J558 V genes and in the intron downstream of the JH4 gene segment.
Naive CD40/LMP1+//CD40−/− B cells had a 0.05% and 0.09% mutation frequency; and naive CD40+/+ B cells, a 0.06% and 0.07% mutation frequency.