Hematopoietic characterization ofPak1−/− mice (age = 8 weeks)
| Parameter . | Pak1+/+* . | Pak1−/−* . | P . |
|---|---|---|---|
| Body weight, n = 16 | 19.6 ± 0.5 | 19.6 ± 0.4 | .924 |
| Peripheral CBC, n = 8 | |||
| HCT, % | 57 ± 0.4 | 59 ± 1.0 | .067 |
| WBC, ×103 | 21 ± 1.2 | 23 ± 1.4 | .468 |
| % Neutrophils | 20 ± 1.5 | 20 ± 0.6 | .888 |
| % Lymphocytes | 70 ± 1.9 | 68 ± 0.8 | .348 |
| Cellularity | |||
| Bone marrow cellularity, ×106 LDMNCs/femur, n = 6 | 9 ± 0.9 | 8 ± 0.9 | .235 |
| Spleen cellularity, ×106 nucleated cells/spleen, n = 5 | 103 ± 4.5 | 104 ± 5.3 | .885 |
| Peritoneal cellularity, ×106 nucleated cells/peritoneum, n = 3 | 4 ± 0.2 | 3 ± 0.1 | .057 |
| Mast cells × 103/peritoneum, n = 3† | 53 ± 5.8 | 40 ± 3.6 | .137 |
| Mast cells/mm2, ear, n = 6‡ | 123 ± 5.6 | 138 ± 6.6 | .112 |
| Parameter . | Pak1+/+* . | Pak1−/−* . | P . |
|---|---|---|---|
| Body weight, n = 16 | 19.6 ± 0.5 | 19.6 ± 0.4 | .924 |
| Peripheral CBC, n = 8 | |||
| HCT, % | 57 ± 0.4 | 59 ± 1.0 | .067 |
| WBC, ×103 | 21 ± 1.2 | 23 ± 1.4 | .468 |
| % Neutrophils | 20 ± 1.5 | 20 ± 0.6 | .888 |
| % Lymphocytes | 70 ± 1.9 | 68 ± 0.8 | .348 |
| Cellularity | |||
| Bone marrow cellularity, ×106 LDMNCs/femur, n = 6 | 9 ± 0.9 | 8 ± 0.9 | .235 |
| Spleen cellularity, ×106 nucleated cells/spleen, n = 5 | 103 ± 4.5 | 104 ± 5.3 | .885 |
| Peritoneal cellularity, ×106 nucleated cells/peritoneum, n = 3 | 4 ± 0.2 | 3 ± 0.1 | .057 |
| Mast cells × 103/peritoneum, n = 3† | 53 ± 5.8 | 40 ± 3.6 | .137 |
| Mast cells/mm2, ear, n = 6‡ | 123 ± 5.6 | 138 ± 6.6 | .112 |
WBC indicates white blood cell; LDMNC, low-density mononuclear cells.
Values represent the mean ± SEM for the number of mice specified for each parameter. Statistical significance compared with the +/+ populations was determined using the Student t test where P value .05 or less.
Mast cells identified by Giemsa stain of cytospins from peritoneal lavage. Total mast cell number calculated by multiplying percentage mast cells × total peritoneal cellularity.
Mast cells identified by toluidine blue stain of histologic ear sections at baseline.