Table 2

Tyrosine phosphorylated peptides identified by PhosphoScan in 6 ALK+ ALCL cell lines (cutoff level, 67%) versus the ALK− cell line Mac-1

Gene symbolpTyr sitePeptideKarpas299SU-DHL1JB-6TSSR-786SUP-M2Mac-1
Kinases          
    ALK 1078* HQELQAMQMELQSPEyK • • • • • •  
    ALK 1092* TSTIMTDyNPNYCFAGK • • • • • •  
    ALK 1096* TSTIMTDYNPNyCFAGK • • • • • •  
    ALK 1131* GLGHGAFGEVyEGQVSGMPNDPSPLQVAVK •   • • •  
    ALK 1278* DIyRASYYR • • • • • •  
    ALK 1282* DIyRASyYR • • • • • •  
    ALK 1283* DIyRASyyR • • • • • •  
    ALK 1507* NKPTSLWNPTyGSWFTEKPTK • • • • • •  
    ALK 1584* HFPCGNVNyGYQQQGLPLEAATAPGAGHyEDTILK • •  •  •  
    ALK 1586* HFPCGNVNYGyQQQGLPLEAATAPGAGHyEDTILK • • • • • •  
    ALK 1604* HFPCGNVNYGyQQQGLPLEAATAPGAGHyEDTILK • • • • • •  
    CDC2 15 IEKIGEGTyGVVYK  • • • • • • 
    CDK3 15 VEKIGEGTyGVVYK • • • • • • • 
    DYRK1A 321 IYQyIQSR • • • • • • • 
    GSK3B 279 GEPNVSyICSR • • • • • • • 
    HIPK1 352 AVCSTyLQSR • •  • • • • 
    MAPK1 187 VADPDHDHTGFLTEyVATR • • • • •  • 
    PGK1 195 ELNyFAK, KELNyFAK • • • •  •  
    PIP5K2B 93 FKEyCPMVFR •   • • •  
    PRPF4B 849 LCDFGSASHVADNDITPyLVSR • • • • • • • 
Metabolism          
    ACLY 682 TTDGVyEGVAIGGDRYPGSTFMDHVLR • • • • • •  
    ATIC 104 VVACNLyPFVK • • • • • •  
    DCP1B 110 LSIyGIWFYDKEECQR • •   • •  
    EIF3S7 318 NLAMEATyINHNFSQQCLR •  • •  •  
    EIF3S9 525 NGDyLCVK • •  • • •  
    ELP3 202 NLHDALSGHTSNNIyEAVK • •  • • •  
    ENO1 43 AAVPSGASTGIyEALELR • • • • • • • 
    ENO1 286 SFIKDyPVVSIEDPFDQDDWGAWQK • •   • •  
    HIST1H4I 51 ISGLIyEETR • •   • •  
    HNRPF 306 ATENDIyNFFSPLNPVR • •   • •  
    HSPCB 483 SIyYITGESKEQVANSAFVER • • • • • •  
    LDHA 238 QVVESAyEVIK • • • • • • • 
    LDHB 239 MVVESAyEVIK • • • • • • • 
    MDH2 56 LTLyDIAHTPGVAADLSHIETK  • • • •   
    NIT2 145 TLSPGDSFSTFDTPyCR • • • • • •  
    NYREN18 126 SKIAETFGLQENyIK •  • • •   
    PABPC1 54 SLGyAYVNFQQPADAER • • • • • •  
    PBEF1 188 YLLETSGNLDGLEyKLHDFGYR • • • • •   
    PKM2 104 TATESFASDPILyRPVAVALDTKGPEIR • • • • • • • 
    PRIM2A 381 IILSNPPSQGDyHGCPFR • • • • •   
    PSMA2 23 LVQIEyALAAVAGGAPSVGIK • • • • •   
    PSMA2 97 KLAQQYyLVYQEPIPTAQLVQR • • • • •   
    PSMB4 102 VNNSTMLGASGDyADFQYLK • •  • • •  
    RNPS1 205 GyAYVEFENPDEAEK • •   • •  
    RPL18A 63 SSGEIVyCGQVFEK • • • • • •  
    RPL31 103 NEDEDSPNKLyTLVTYVPVTTFK • • • • • •  
    RPL7 139 IVEPyIAWGYPNLK •  • • • •  
    RPLP0 24 IIQLLDDyPKCFIVGADNVGSK • • • • • • • 
    RPS13 37 LTSDDVKEQIyK • •  •  •  
    TXNRD1 11 SyDYDLIIIGGGSGGLAAAK • • • •    
    TXNRD1 13 SYDyDLIIIGGGSGGLAAAK • • • • • •  
    WDR54 33 NLTyFGVVHGPSAQLLSAAPEGVPLAQR • •   • •  
G-proteins          
    GDI2 203 TDDYLDQPCyETINR • •  •  • • 
    RASA1 615 HFTNPyCNIYLNSVQVAK • • • • • •  
Adaptor proteins          
    GRLF1 1105 NEEENIySVPHDSTQGK  •  • •   
    HSPA4 336 LKKEDIyAVEIVGGATR • •  • • •  
    HSPD1 227 GYISPyFINTSK • •   • •  
    IRS1 46 AASEAGGPARLEyYENEKK  •  • •   
    SHC1 427 ELFDDPSyVNVQNLDK • • • • • • • 
    TRAP1 498 NIyYLCAPNR • • • • • •  
Transcription factors          
    EEF1A1 141 EHALLAyTLGVK • • • • •  • 
    STAT3 704 YCRPESQEHPEADPGAAPyLK • • • • • • • 
    STAT3 705 YCRPESQEHPEADPGSAAPyLK • • • • • • • 
Cytoskeleton          
    ACTB 218 DIKEKLCyVALDFEQEMATAASSSSLEK • •  • •   
    DNCH1 3379 NYMSNPSYNyEIVNR • • •  • •  
    STOML2 124 ASYGVEDPEyAVTQLAQTTMR • •  • •   
    VASP 38 VQIyHNPTANSFR • • • • •   
    VIM 116 TNEKVELQELNDRFANyIDKVR, FANyIDKVR •   • • •  
    WDR1 238 AHDGGIyAISWSPDSTHLLSASGDKTSK • •  • • •  
Gene symbolpTyr sitePeptideKarpas299SU-DHL1JB-6TSSR-786SUP-M2Mac-1
Kinases          
    ALK 1078* HQELQAMQMELQSPEyK • • • • • •  
    ALK 1092* TSTIMTDyNPNYCFAGK • • • • • •  
    ALK 1096* TSTIMTDYNPNyCFAGK • • • • • •  
    ALK 1131* GLGHGAFGEVyEGQVSGMPNDPSPLQVAVK •   • • •  
    ALK 1278* DIyRASYYR • • • • • •  
    ALK 1282* DIyRASyYR • • • • • •  
    ALK 1283* DIyRASyyR • • • • • •  
    ALK 1507* NKPTSLWNPTyGSWFTEKPTK • • • • • •  
    ALK 1584* HFPCGNVNyGYQQQGLPLEAATAPGAGHyEDTILK • •  •  •  
    ALK 1586* HFPCGNVNYGyQQQGLPLEAATAPGAGHyEDTILK • • • • • •  
    ALK 1604* HFPCGNVNYGyQQQGLPLEAATAPGAGHyEDTILK • • • • • •  
    CDC2 15 IEKIGEGTyGVVYK  • • • • • • 
    CDK3 15 VEKIGEGTyGVVYK • • • • • • • 
    DYRK1A 321 IYQyIQSR • • • • • • • 
    GSK3B 279 GEPNVSyICSR • • • • • • • 
    HIPK1 352 AVCSTyLQSR • •  • • • • 
    MAPK1 187 VADPDHDHTGFLTEyVATR • • • • •  • 
    PGK1 195 ELNyFAK, KELNyFAK • • • •  •  
    PIP5K2B 93 FKEyCPMVFR •   • • •  
    PRPF4B 849 LCDFGSASHVADNDITPyLVSR • • • • • • • 
Metabolism          
    ACLY 682 TTDGVyEGVAIGGDRYPGSTFMDHVLR • • • • • •  
    ATIC 104 VVACNLyPFVK • • • • • •  
    DCP1B 110 LSIyGIWFYDKEECQR • •   • •  
    EIF3S7 318 NLAMEATyINHNFSQQCLR •  • •  •  
    EIF3S9 525 NGDyLCVK • •  • • •  
    ELP3 202 NLHDALSGHTSNNIyEAVK • •  • • •  
    ENO1 43 AAVPSGASTGIyEALELR • • • • • • • 
    ENO1 286 SFIKDyPVVSIEDPFDQDDWGAWQK • •   • •  
    HIST1H4I 51 ISGLIyEETR • •   • •  
    HNRPF 306 ATENDIyNFFSPLNPVR • •   • •  
    HSPCB 483 SIyYITGESKEQVANSAFVER • • • • • •  
    LDHA 238 QVVESAyEVIK • • • • • • • 
    LDHB 239 MVVESAyEVIK • • • • • • • 
    MDH2 56 LTLyDIAHTPGVAADLSHIETK  • • • •   
    NIT2 145 TLSPGDSFSTFDTPyCR • • • • • •  
    NYREN18 126 SKIAETFGLQENyIK •  • • •   
    PABPC1 54 SLGyAYVNFQQPADAER • • • • • •  
    PBEF1 188 YLLETSGNLDGLEyKLHDFGYR • • • • •   
    PKM2 104 TATESFASDPILyRPVAVALDTKGPEIR • • • • • • • 
    PRIM2A 381 IILSNPPSQGDyHGCPFR • • • • •   
    PSMA2 23 LVQIEyALAAVAGGAPSVGIK • • • • •   
    PSMA2 97 KLAQQYyLVYQEPIPTAQLVQR • • • • •   
    PSMB4 102 VNNSTMLGASGDyADFQYLK • •  • • •  
    RNPS1 205 GyAYVEFENPDEAEK • •   • •  
    RPL18A 63 SSGEIVyCGQVFEK • • • • • •  
    RPL31 103 NEDEDSPNKLyTLVTYVPVTTFK • • • • • •  
    RPL7 139 IVEPyIAWGYPNLK •  • • • •  
    RPLP0 24 IIQLLDDyPKCFIVGADNVGSK • • • • • • • 
    RPS13 37 LTSDDVKEQIyK • •  •  •  
    TXNRD1 11 SyDYDLIIIGGGSGGLAAAK • • • •    
    TXNRD1 13 SYDyDLIIIGGGSGGLAAAK • • • • • •  
    WDR54 33 NLTyFGVVHGPSAQLLSAAPEGVPLAQR • •   • •  
G-proteins          
    GDI2 203 TDDYLDQPCyETINR • •  •  • • 
    RASA1 615 HFTNPyCNIYLNSVQVAK • • • • • •  
Adaptor proteins          
    GRLF1 1105 NEEENIySVPHDSTQGK  •  • •   
    HSPA4 336 LKKEDIyAVEIVGGATR • •  • • •  
    HSPD1 227 GYISPyFINTSK • •   • •  
    IRS1 46 AASEAGGPARLEyYENEKK  •  • •   
    SHC1 427 ELFDDPSyVNVQNLDK • • • • • • • 
    TRAP1 498 NIyYLCAPNR • • • • • •  
Transcription factors          
    EEF1A1 141 EHALLAyTLGVK • • • • •  • 
    STAT3 704 YCRPESQEHPEADPGAAPyLK • • • • • • • 
    STAT3 705 YCRPESQEHPEADPGSAAPyLK • • • • • • • 
Cytoskeleton          
    ACTB 218 DIKEKLCyVALDFEQEMATAASSSSLEK • •  • •   
    DNCH1 3379 NYMSNPSYNyEIVNR • • •  • •  
    STOML2 124 ASYGVEDPEyAVTQLAQTTMR • •  • •   
    VASP 38 VQIyHNPTANSFR • • • • •   
    VIM 116 TNEKVELQELNDRFANyIDKVR, FANyIDKVR •   • • •  
    WDR1 238 AHDGGIyAISWSPDSTHLLSASGDKTSK • •  • • •  
*

The indicated ALK pTyr sites refer to the full-length ALK receptor, and they correspond to NPM-ALK pTyr sites 138, 152, 156, 191, 338, 342, 343, 567, 644, 646, and 664, respectively

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