Effect of IGF-1 on expression of CXCL12 and CCL25 in thymic epithelial cells
| Day of IGF-1 infusion . | Relative expression, IGF/diluent . | |
|---|---|---|
| CXCL12 . | CCL25 . | |
| Cortical TECs (CD45−, Ly51+, UEA1−) | ||
| 4 | 3.20 (2.75-3.73) | 1.92 (1.26-2.93) |
| 7 | 2.19 (1.78-2.69) | 2.04 (1.38-2.50) |
| Medullary TECs (CD45−, Ly51−, UEA1+) | ||
| 4 | 7.18 (4.00-15.14) | 1.85 (1.51-2.77) |
| 7 | 1.44 (0.92-2.21) | 0.51 (0.38-0.69) |
| Day of IGF-1 infusion . | Relative expression, IGF/diluent . | |
|---|---|---|
| CXCL12 . | CCL25 . | |
| Cortical TECs (CD45−, Ly51+, UEA1−) | ||
| 4 | 3.20 (2.75-3.73) | 1.92 (1.26-2.93) |
| 7 | 2.19 (1.78-2.69) | 2.04 (1.38-2.50) |
| Medullary TECs (CD45−, Ly51−, UEA1+) | ||
| 4 | 7.18 (4.00-15.14) | 1.85 (1.51-2.77) |
| 7 | 1.44 (0.92-2.21) | 0.51 (0.38-0.69) |
Real-time quantitative RT-PCR was performed from cDNA isolated from sorted thymic epithelial cells pooled from 3 mice per group per time point of IGF-1 versus diluent controls using primer/probe sets specific for CXCL12, CCL25, and GAPDH as the endogenous control. Data presented are the means (with ranges in parentheses) of relative expression of CXCL12 and CCL25 in the specified TEC population between IGF-1– and diluent-treated mice at day 4 and day 7 of diluent/IGF-1 administration. Values were determined using the comparative CT method.