RT-PCR primers used
| Primer name . | Direction . | Application . | Sequence, 5′-3′ . |
|---|---|---|---|
| Actin | Sense | RT-PCR | ATCTGGCACCACACCTTCTACAATGAGCTGCG |
| Actin | Antisense | RT-PCR | CGTCATACTCCTGCTTGCTGATCCACATCTGC |
| CD122 | Sense | RT-PCR | CCCAGTTCACATGCTTCTAC |
| CD122 | Antisense | RT-PCR | GACAACTTGGAGGGAGATG |
| E2A | Sense | RT-PCR | CGAGGAGAACACGTCAGCGG |
| E2A | Antisense | RT-PCR | GCTCGGCCTTCTGCTCTG |
| ID1 | Sense | RT-PCR | GCTCATCGACTACATCAGGG |
| ID1 | Antisense | RT-PCR | CACAGAGCACGTAATTCCTC |
| ID2 | Sense | RT-PCR | TCAGCCTGCATCACCAGAGA |
| ID2 | Antisense | RT-PCR | CCATTCAACTTGTCCTCC |
| ID3 | Sense | RT-PCR | AGGGAAGGGCCCGGCAGCTG |
| ID3 | Antisense | RT-PCR | TTCCGGCAGGAGAGGTTCCC |
| ID4 | Sense | RT-PCR | ATGGGATGAGGAAATGCTTG |
| ID4 | Antisense | RT-PCR | TGGAGGAAGGAAAGCAGAAA |
| IL-7R | Sense | RT-PCR | GTCAACATCACCAATCTGG |
| IL-7R | Antisense | RT-PCR | GTAAGATAGGATCCATCTCC |
| Notch-1 | Sense | RT-PCR | CACTGTGGGCGGGTCC |
| Notch-1 | Antisense | RT-PCR | GTTGTATTGGTTCGGCACCAT |
| RAG-1 | Sense | RT-PCR | GAGCAAGGTACCTCAGCCAG |
| RAG-1 | Antisense | RT-PCR | AACAATGGCTGAGTTGGGAC |
| SCF | Sense | RT-PCR | CTACGAGATATGGTAATACAA |
| SCF | Antisense | RT-PCR | ATCGCTACTGCTGTCATTC |
| Slug | Sense | RT-PCR | AAAAAGCCAAACTACAGCGAAC |
| Slug | Antisense | RT-PCR | GAGGTGTCAGATGGAGGAGGG |
| Actin | Sense | Q-RT-PCR | TACCTCATGAAGATCCTCA |
| Actin | Antisense | Q-RT-PCR | TTCGTGGATGCCACAGGAC |
| ID2 | Sense | Q-RT-PCR | TCAGCCTGCATCACCAGAGA |
| ID2 | Antisense | Q-RT-PCR | CCATTCAACTTGTCCTCC |
| ID3 | Sense | Q-RT-PCR | TCGGAACGCAGTCTGGCCATC |
| ID3 | Antisense | Q-RT-PCR | CTCGGCTGTCTGGATGGGAAG |
| Primer name . | Direction . | Application . | Sequence, 5′-3′ . |
|---|---|---|---|
| Actin | Sense | RT-PCR | ATCTGGCACCACACCTTCTACAATGAGCTGCG |
| Actin | Antisense | RT-PCR | CGTCATACTCCTGCTTGCTGATCCACATCTGC |
| CD122 | Sense | RT-PCR | CCCAGTTCACATGCTTCTAC |
| CD122 | Antisense | RT-PCR | GACAACTTGGAGGGAGATG |
| E2A | Sense | RT-PCR | CGAGGAGAACACGTCAGCGG |
| E2A | Antisense | RT-PCR | GCTCGGCCTTCTGCTCTG |
| ID1 | Sense | RT-PCR | GCTCATCGACTACATCAGGG |
| ID1 | Antisense | RT-PCR | CACAGAGCACGTAATTCCTC |
| ID2 | Sense | RT-PCR | TCAGCCTGCATCACCAGAGA |
| ID2 | Antisense | RT-PCR | CCATTCAACTTGTCCTCC |
| ID3 | Sense | RT-PCR | AGGGAAGGGCCCGGCAGCTG |
| ID3 | Antisense | RT-PCR | TTCCGGCAGGAGAGGTTCCC |
| ID4 | Sense | RT-PCR | ATGGGATGAGGAAATGCTTG |
| ID4 | Antisense | RT-PCR | TGGAGGAAGGAAAGCAGAAA |
| IL-7R | Sense | RT-PCR | GTCAACATCACCAATCTGG |
| IL-7R | Antisense | RT-PCR | GTAAGATAGGATCCATCTCC |
| Notch-1 | Sense | RT-PCR | CACTGTGGGCGGGTCC |
| Notch-1 | Antisense | RT-PCR | GTTGTATTGGTTCGGCACCAT |
| RAG-1 | Sense | RT-PCR | GAGCAAGGTACCTCAGCCAG |
| RAG-1 | Antisense | RT-PCR | AACAATGGCTGAGTTGGGAC |
| SCF | Sense | RT-PCR | CTACGAGATATGGTAATACAA |
| SCF | Antisense | RT-PCR | ATCGCTACTGCTGTCATTC |
| Slug | Sense | RT-PCR | AAAAAGCCAAACTACAGCGAAC |
| Slug | Antisense | RT-PCR | GAGGTGTCAGATGGAGGAGGG |
| Actin | Sense | Q-RT-PCR | TACCTCATGAAGATCCTCA |
| Actin | Antisense | Q-RT-PCR | TTCGTGGATGCCACAGGAC |
| ID2 | Sense | Q-RT-PCR | TCAGCCTGCATCACCAGAGA |
| ID2 | Antisense | Q-RT-PCR | CCATTCAACTTGTCCTCC |
| ID3 | Sense | Q-RT-PCR | TCGGAACGCAGTCTGGCCATC |
| ID3 | Antisense | Q-RT-PCR | CTCGGCTGTCTGGATGGGAAG |