Table 3 Engraftment data for human... | American Society of Hematology

Table 3

Engraftment data for human lymphomyeloid progenitor transplants assessed by FACS

Mouse ID	Vector	CID	Day post BMT	% huCD45⁺ cells	%CD34⁺Lin⁻/huCD45⁺	Fold increase CD34⁺Lin⁻ human cells
Experiment A
1	F36V-MPL	+	35	22.4	46	215.3
2	F36V-MPL	+	49	52.8	36.1	345
3	F36V-MPL	−	35	0.71	0.1	0.02
4	F36V-MPL	−	49	0	0	0
5	F36V-MPL	−	49	0.05	0	0
6	Control (no transplant)		49	0	0	0
Experiment B
1	F36V-MPL	+	49	21.9	23.2	75.7
2	F36V-MPL	+	49	26.1	13.1	31.8
3	F36V-MPL	−	49	0.76	20.6	0.6
4	F36V-MPL	−	49	0.83	18.3	0.5
5	F36V-MPL	−	49	0	0	0
6	F36V	−	49	0.64	22.2	0.5
7	F36V	−	49	0	0	0
8	Control (no transplant)		49	0	0	0
Summary				Mean % human CD45⁺ cells	Mean %CD34⁺Lin⁻/huCD45⁺ cells	Mean fold increase CD34⁺Lin⁻ human cells
	F36V-MPL	+		30.8	29.6	167
	F36V-MPL	−		0.4	6.5	0.2

Mouse ID	Vector	CID	Day post BMT	% huCD45⁺ cells	%CD34⁺Lin⁻/huCD45⁺	Fold increase CD34⁺Lin⁻ human cells
Experiment A
1	F36V-MPL	+	35	22.4	46	215.3
2	F36V-MPL	+	49	52.8	36.1	345
3	F36V-MPL	−	35	0.71	0.1	0.02
4	F36V-MPL	−	49	0	0	0
5	F36V-MPL	−	49	0.05	0	0
6	Control (no transplant)		49	0	0	0
Experiment B
1	F36V-MPL	+	49	21.9	23.2	75.7
2	F36V-MPL	+	49	26.1	13.1	31.8
3	F36V-MPL	−	49	0.76	20.6	0.6
4	F36V-MPL	−	49	0.83	18.3	0.5
5	F36V-MPL	−	49	0	0	0
6	F36V	−	49	0.64	22.2	0.5
7	F36V	−	49	0	0	0
8	Control (no transplant)		49	0	0	0
Summary				Mean % human CD45⁺ cells	Mean %CD34⁺Lin⁻/huCD45⁺ cells	Mean fold increase CD34⁺Lin⁻ human cells
	F36V-MPL	+		30.8	29.6	167
	F36V-MPL	−		0.4	6.5	0.2

Fold expansion of human CD34⁺Lin⁻ cells is derived from the percentage of huCD45⁺CD34⁺Lin⁻ cells and total cells harvested from bone marrow (femurs, tibias) divided by the number of CD34⁺CD38⁻Lin⁻CD7⁻ human cells transplanted (n = 14 mice, 2 independent experiments). Fold expansion of F36V-MPL–transduced CD34⁺Lin⁻ cells in CID-treated group is significantly (P = .004) greater compared with all other groups.

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