Specificities of lyases
| Lyase (reference) . | Substrate(s) . | Glycosidic linkage(s) cleaved . |
|---|---|---|
| Chondroitin B-lyase (Michelacci et al21 ) | DS | GalNAc4SO3→IdoA |
| Chondroitin ABC-lyase (Oike et al22 ) | DS, C4S, C6S | GalNAc4SO3 and/or 6SO3→UA;GalNAc4SO3→UA2SO3 |
| Flavobacterium heparitinase (Linker and Hovingh23 ) | HS | GlcNAc6SO3→UA;GlcNSO3→UA;GlcNSO36SO3→UA |
| Lyase (reference) . | Substrate(s) . | Glycosidic linkage(s) cleaved . |
|---|---|---|
| Chondroitin B-lyase (Michelacci et al21 ) | DS | GalNAc4SO3→IdoA |
| Chondroitin ABC-lyase (Oike et al22 ) | DS, C4S, C6S | GalNAc4SO3 and/or 6SO3→UA;GalNAc4SO3→UA2SO3 |
| Flavobacterium heparitinase (Linker and Hovingh23 ) | HS | GlcNAc6SO3→UA;GlcNSO3→UA;GlcNSO36SO3→UA |
C4S indicates chondroitin 4-sulfate; C6S, chondroitin 6-sulfate; GalNAc4SO3, N-acetylgalactosamine 4-O-sulfate; GlcNAc6SO3, N-acetylglucosamine 6-O-sulfate; GlcNSO3, N-sulfoglucosamine; GlcNSO36SO3, N-sulfoglucosamine 6-O-sulfate; IdoA, iduronic acid; and UA, hexuronic acid (glucuronic or iduronic acid).