HLA class I typing of BLCL target cell panel
| ID . | Short ID . | HLA-B . | AA 80 . | Bw4/6 . | HLA-A . | HLA-C . | C1/2 . |
|---|---|---|---|---|---|---|---|
| IHW 9084 | B13 | 1302 | T | 4 | 3001 | 0602 | 2 |
| IHW 9067 | B27 | 2705 | T | 4 | 0201 | 0102 | 1 |
| IHW 9009 | B37 | 3701 | T | 4 | 0101 | 0602 | 2 |
| IHW 9090 | B4402 | 4402 | T | 4 | 0201 | 0501 | 2 |
| IHW 9027 | B4403 | 4403 | T | 4 | 2902 | 1601 | 1 |
| IHW 9047 | B47 | 4701 | T | 4 | 0301 | 0602 | 2 |
| IHW 9062 | B38 | 3801 | I | 4 | 0201 | 1203 | 1 |
| IHW 9040 | B49 | 4901 | I | 4 | 0101 | 0701 | 1 |
| IHW 9016 | B51 | 5101 | I | 4 | 0204 | 1502 | 2 |
| IHW 9011 | B52 | 5201 | I | 4 | 0101 | 1202 | 1 |
| IHW 9010 | B53 | 5301 | I | 4 | 6802 | 0401 | 2 |
| IHW 9052 | B57 | 5701 | I | 4 | 0201 | 0602 | 2 |
| IHW 9157 | B58 | 5801 | I | 4 | 33 | 0302 | 1 |
| IHW 9029 | A23 | 1402 | I | 6 | 2301 | 0802 | 1 |
| IHW 9001 | A24 | 0702 | I | 6 | 2402 | 0702 | 1 |
| IHW 9008 | A25 | 1801 | I | 6 | 2501 | 1203 | 1 |
| Q94 0055016Y | A32 | 1501, 3901 | I | 6,6 | 3201, 0201 | 0303, 1203 | 1,1 |
| IHW 9065 | B07 | 0702 | N | 6 | 0301 | 0702 | 1 |
| IHW 9088 | B08 | 0801 | N | 6 | 0101 | 0701 | 1 |
| IHW 9029 | B14 | 1402 | N | 6 | 2301 | 0802 | 1 |
| IHW 9099 | B15 | 1501 | N | 6 | 0217 | 0303 | 1 |
| IHW 9019 | B18 | 1801 | N | 6 | 3002 | 0501 | 2 |
| IHW 9068 | B35 | 3501 | N | 6 | 0201 | 0401 | 2 |
| Q94 0052722Q | B39 | 0702, 3901 | N | 6,6 | 0201, 0301 | 0702, 1203 | 1,1 |
| IHW 9084 | B40 | 4002 | N | 6 | 0201 | 0202 | 2 |
| IHW 9043 | B41 | 4101 | N | 6 | 0101 | 1701 | 2 |
| IHW 9021 | B42 | 4201 | N | 6 | 3001, 6802 | 1701 | 2 |
| IHW 9058 | B45 | 4501 | N | 6 | 0201 | 1601 | 1 |
| IHW 9076 | B46 | 4601 | N | 6 | 0206, 0207 | 0102, 0801 | 1,1 |
| R98 0903165W | B50 | 5001, 0702 | N | 6,6 | 0301, 0201 | — | — |
| R04 0901000J | B55 | 5502, 0801 | N | 6,6 | 0101, 0206 | 0102, 0701 | 1,1 |
| R97 0330581F | B56 | 5601, 0801 | N | 6,6 | 01, 34 | — | — |
| ID . | Short ID . | HLA-B . | AA 80 . | Bw4/6 . | HLA-A . | HLA-C . | C1/2 . |
|---|---|---|---|---|---|---|---|
| IHW 9084 | B13 | 1302 | T | 4 | 3001 | 0602 | 2 |
| IHW 9067 | B27 | 2705 | T | 4 | 0201 | 0102 | 1 |
| IHW 9009 | B37 | 3701 | T | 4 | 0101 | 0602 | 2 |
| IHW 9090 | B4402 | 4402 | T | 4 | 0201 | 0501 | 2 |
| IHW 9027 | B4403 | 4403 | T | 4 | 2902 | 1601 | 1 |
| IHW 9047 | B47 | 4701 | T | 4 | 0301 | 0602 | 2 |
| IHW 9062 | B38 | 3801 | I | 4 | 0201 | 1203 | 1 |
| IHW 9040 | B49 | 4901 | I | 4 | 0101 | 0701 | 1 |
| IHW 9016 | B51 | 5101 | I | 4 | 0204 | 1502 | 2 |
| IHW 9011 | B52 | 5201 | I | 4 | 0101 | 1202 | 1 |
| IHW 9010 | B53 | 5301 | I | 4 | 6802 | 0401 | 2 |
| IHW 9052 | B57 | 5701 | I | 4 | 0201 | 0602 | 2 |
| IHW 9157 | B58 | 5801 | I | 4 | 33 | 0302 | 1 |
| IHW 9029 | A23 | 1402 | I | 6 | 2301 | 0802 | 1 |
| IHW 9001 | A24 | 0702 | I | 6 | 2402 | 0702 | 1 |
| IHW 9008 | A25 | 1801 | I | 6 | 2501 | 1203 | 1 |
| Q94 0055016Y | A32 | 1501, 3901 | I | 6,6 | 3201, 0201 | 0303, 1203 | 1,1 |
| IHW 9065 | B07 | 0702 | N | 6 | 0301 | 0702 | 1 |
| IHW 9088 | B08 | 0801 | N | 6 | 0101 | 0701 | 1 |
| IHW 9029 | B14 | 1402 | N | 6 | 2301 | 0802 | 1 |
| IHW 9099 | B15 | 1501 | N | 6 | 0217 | 0303 | 1 |
| IHW 9019 | B18 | 1801 | N | 6 | 3002 | 0501 | 2 |
| IHW 9068 | B35 | 3501 | N | 6 | 0201 | 0401 | 2 |
| Q94 0052722Q | B39 | 0702, 3901 | N | 6,6 | 0201, 0301 | 0702, 1203 | 1,1 |
| IHW 9084 | B40 | 4002 | N | 6 | 0201 | 0202 | 2 |
| IHW 9043 | B41 | 4101 | N | 6 | 0101 | 1701 | 2 |
| IHW 9021 | B42 | 4201 | N | 6 | 3001, 6802 | 1701 | 2 |
| IHW 9058 | B45 | 4501 | N | 6 | 0201 | 1601 | 1 |
| IHW 9076 | B46 | 4601 | N | 6 | 0206, 0207 | 0102, 0801 | 1,1 |
| R98 0903165W | B50 | 5001, 0702 | N | 6,6 | 0301, 0201 | — | — |
| R04 0901000J | B55 | 5502, 0801 | N | 6,6 | 0101, 0206 | 0102, 0701 | 1,1 |
| R97 0330581F | B56 | 5601, 0801 | N | 6,6 | 01, 34 | — | — |
HLA types of target cells used in flow cytometric and chromium release assays. In all cases, HLA alleles were assigned on the basis of sequencing of exons 2 and 3. In some cases, the assignment of class I HLA alleles is based on the most common allele consistent with the sequence in exons 2 and 3, and alleles differing in other exons cannot be excluded. Targets that were homozygous for HLA-B50, HLA-B55, and HLA-B56 were not available. Therefore, cells that were heterozygous for these antigens and had a common Bw6 antigen as the second antigen were selected. AA80 indicates the amino acid present at residue 80 of the HLA-B alleles.
— indicates HLA-C typing not performed.