HLA class I and KIR3DL1 allele typing of NK-cell donors
| Donor . | HLA-A . | HLA-B . | Bw4/6 . | HLA-C . | C1/2 . | KIR3DL1 typing . | KIR3DL1 expression . |
|---|---|---|---|---|---|---|---|
| 1 | 0201, 2902 | 4403, 5703 | 4 | 0701, 1601 | 1 | 01501/017, 008 | High |
| 2 | 0201, 2601 | 0702, 3801 | 4,6 | 0702, 1203 | 1 | 002, 00101 | High |
| 3 | 0101, 2402 | 0801, 5109 | 4,6 | 0102, 0701 | 1 | 00501-like, 01502 | High, low |
| 4 | 0201, 3303 | 4601, 5801 | 4,6 | 0102, 0302 | 1 | 00501, 01502 | High, low |
| 5 | 1101 | 1301, 1501 | 4,6 | 0304, 0401 | 1,2 | 007, 01502 | High, low |
| 6 | 1101 | 1301, 1501 | 4,6 | 0304, 0401 | 1,2 | 00501, 007 | Low |
| 7 | 0301, 3101 | 1302, 3501 | 4,6 | 0401, 0602 | 2 | 001/016, 002 | High |
| 8 | 0101, 0301 | 0801 | 6 | 0701 | 1 | NT | High |
| 9 | 0201, 1101 | 1501, 3501 | 6 | 0303, 0401 | 1,2 | NT | Low |
| 10 | 0101, 2402 | 0801, 5501 | 6 | 0303, 0701 | 1 | NT | High |
| Donor . | HLA-A . | HLA-B . | Bw4/6 . | HLA-C . | C1/2 . | KIR3DL1 typing . | KIR3DL1 expression . |
|---|---|---|---|---|---|---|---|
| 1 | 0201, 2902 | 4403, 5703 | 4 | 0701, 1601 | 1 | 01501/017, 008 | High |
| 2 | 0201, 2601 | 0702, 3801 | 4,6 | 0702, 1203 | 1 | 002, 00101 | High |
| 3 | 0101, 2402 | 0801, 5109 | 4,6 | 0102, 0701 | 1 | 00501-like, 01502 | High, low |
| 4 | 0201, 3303 | 4601, 5801 | 4,6 | 0102, 0302 | 1 | 00501, 01502 | High, low |
| 5 | 1101 | 1301, 1501 | 4,6 | 0304, 0401 | 1,2 | 007, 01502 | High, low |
| 6 | 1101 | 1301, 1501 | 4,6 | 0304, 0401 | 1,2 | 00501, 007 | Low |
| 7 | 0301, 3101 | 1302, 3501 | 4,6 | 0401, 0602 | 2 | 001/016, 002 | High |
| 8 | 0101, 0301 | 0801 | 6 | 0701 | 1 | NT | High |
| 9 | 0201, 1101 | 1501, 3501 | 6 | 0303, 0401 | 1,2 | NT | Low |
| 10 | 0101, 2402 | 0801, 5501 | 6 | 0303, 0701 | 1 | NT | High |
The KIR3DL1*005-like allele in donor 3 differs from KIR3DL1*005 by a valine-to-leucine substitution at amino acid 18 in the D0 domain. In all cases, HLA alleles were assigned on the basis of sequencing of exons 2 and 3. In some cases, the assignment of class I HLA alleles is based on the most common allele consistent with the sequence in exons 2 and 3, and alleles differing in other exons cannot be excluded. As the signal peptide of KIR3DL1 was not sequenced, KIR3DL1*01501 cannot be distinguished from KIR3DL1*017 in donor 1, and KIR*001 cannot be distinguished from KIR3DL1*016 in donor 7.
NT indicates not tested.