Peritoneal exudate cellularity in mixed bone marrow chimeras
Group . | Donor mice . | Cell genotype . | Peritoneal subpopulation, cell no., ×107 . | ||||
---|---|---|---|---|---|---|---|
B cells . | T cells . | B1 . | B2 . | B1a . | B1b . | ||
A | Itch−/− | −/− | −/− | 0.79* | 0.24* | 0.05* | 0.65* |
B | B6 CD45.1 + B10 | WT | WT | 0.45 | 0.45 | 0.11 | 0.29 |
C | μMT + Itch−/−TCRa−/− | −/− | WT | 0.27 | 0.33* | 0.05* | 0.20* |
D | TCRa−/− CD45.1 + Itch−/− | WT & −/− | −/− | 0.62* | 0.24* | 0.05* | 0.51 |
E | B6 CD45.1 + Itch−/−TCRa−/− | WT & −/− | WT | 0.32 | 0.62 | 0.05* | 0.23 |
F | B6 CD45.1 + Itch−/− | WT & −/− | WT & −/− | 0.75* | 0.17* | 0.06* | 0.65* |
Group . | Donor mice . | Cell genotype . | Peritoneal subpopulation, cell no., ×107 . | ||||
---|---|---|---|---|---|---|---|
B cells . | T cells . | B1 . | B2 . | B1a . | B1b . | ||
A | Itch−/− | −/− | −/− | 0.79* | 0.24* | 0.05* | 0.65* |
B | B6 CD45.1 + B10 | WT | WT | 0.45 | 0.45 | 0.11 | 0.29 |
C | μMT + Itch−/−TCRa−/− | −/− | WT | 0.27 | 0.33* | 0.05* | 0.20* |
D | TCRa−/− CD45.1 + Itch−/− | WT & −/− | −/− | 0.62* | 0.24* | 0.05* | 0.51 |
E | B6 CD45.1 + Itch−/−TCRa−/− | WT & −/− | WT | 0.32 | 0.62 | 0.05* | 0.23 |
F | B6 CD45.1 + Itch−/− | WT & −/− | WT & −/− | 0.75* | 0.17* | 0.06* | 0.65* |
Bone marrow cells from indicated donor mice were depleted from B220+ and Thy1.2+ cells and injected in a 1:1 ratio to a total of 6×106 cells per mouse (excluding group A where 3×106 cells per mouse were injected). Numbers of peritoneal exudate cells of mice are presented 8 to 10 weeks after adoptive transfer (n = 5).
P < .05 in t test with group B.