Putative markers for undifferentiated MSCs and their differentiated progeny identified from ANOVA and post hoc analysis
| Probe ID . | Gene symbol . | Description . | Entrez Gene ID . | Sample ID . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| M1 . | A07 . | A14 . | C07 . | C14 . | O07 . | O14 . | ||||
| MSC markers | ||||||||||
| GI_21264358-I | MASP1 | Homo sapiens mannan-binding lectin serine protease 1 (C4/C2 activating component of Ra-reactive factor) (MASP1), transcript variant 2, mRNA | 5648 | 2.1247 | −0.1619 | 0.0000 | −0.9388 | 0.0000 | 0.2726 | 0.0000 |
| GI_5803016-S | FOSB | H sapiens FBJ murine osteosarcoma viral oncogene homolog B (FOSB), mRNA | 2354 | 3.0524 | −0.3024 | 1.8793 | 0.5454 | −1.7003 | −1.1363 | −0.0643 |
| Adipogenic markers | ||||||||||
| GI_12669906-S | IGFBP6 | H sapiens acyl-CoA synthetase long-chain family member 1 (ACSL1), mRNA | 2180 | −0.1096 | 1.3134 | 1.8922 | −0.1455 | −0.2354 | 0.0038 | 0.0985 |
| GI_20070269-S | G0S2 | H sapiens putative lymphocyte G0/G1 switch gene (G0S2), mRNA | 50486 | −0.4908 | 2.8306 | 3.2601 | 0.4378 | 0.8131 | −0.7489 | −0.5906 |
| GI_22027484-S | >RASD1 | H sapiens RAS, dexamethasone-induced 1 (RASD1), mRNA | 51655 | 0.8445 | 2.2295 | 1.7099 | −0.7705 | −3.3403 | −0.3931 | −0.1897 |
| GI_31377551-S | RAP2A | H sapiens RAP2A, member of RAS oncogene family (RAP2A), mRNA | 5911 | −0.2610 | 1.9745 | 2.3584 | 0.0316 | −0.7797 | −0.1838 | −0.0538 |
| GI_34222182-S | DDIT4L | H sapiens DNA-damage-inducible transcript 4-like (DDIT4L), mRNA | 115265 | −0.8135 | 1.9974 | 2.2798 | 0.6702 | 0.3898 | −2.5613 | −0.3804 |
| GI_45446741-S | AKR1C2 | H sapiens aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III) (AKR1C2), transcript variant 1, mRNA | 1646 | −0.3467 | 2.1801 | 2.4928 | 0.1962 | −0.0946 | −0.4847 | 0.0891 |
| Chondrogenic markers | ||||||||||
| GI_13324676-S | ENPP1 | H sapiens ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), mRNA | 5167 | 0.2188 | −0.7887 | −0.5631 | 1.9896 | 3.0516 | −1.1652 | −0.4323 |
| GI_17981693-A | CDKN2B | H sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1, mRNA | 1030 | 0.3149 | −0.0605 | −0.0050 | 1.8528 | 1.8740 | −0.8466 | −0.9401 |
| GI_19923197-S | RUNX1 | H sapiens runt-related transcription factor 1 (acute myeloid leukemia 1; aml1 oncogene; RUNX1), mRNA | 861 | 0.0426 | 0.0033 | −0.0123 | 1.6058 | 2.0494 | −0.7632 | −0.3739 |
| GI_23111004-S | MFAP4 | H sapiens microfibrillar-associated protein 4 (MFAP4), mRNA | 4239 | −0.2909 | −0.5414 | −1.7318 | 2.1228 | 1.7113 | 0.0428 | 0.6477 |
| GI_24430217-S | IL11 | H sapiens interleukin 11 (IL11), mRNA | 3589 | 0.4145 | −1.0965 | −0.7898 | 2.0906 | 2.4231 | −1.7893 | −1.0528 |
| GI_24475846-S | IVNS1ABP | H sapiens influenza virus NS1A binding protein (IVNS1ABP), mRNA | 10625 | −0.2214 | −0.4830 | −0.4939 | 1.4905 | 2.2335 | 0.4409 | 0.3169 |
| GI_29568085-S | SDC1 | H sapiens syndecan 1 (SDC1), mRNA | 6382 | 0.6379 | −1.7792 | −1.7169 | 2.0289 | 2.5978 | −1.7140 | −1.2265 |
| GI_32964829-S | COL8A2 | H sapiens collagen, type VIII, alpha 2 (COL8A2), mRNA | 1296 | −0.4979 | −0.1827 | −0.1771 | 2.3983 | 1.1285 | −0.1501 | 0.5740 |
| GI_34304351-S | BGN | H sapiens biglycan (BGN), mRNA | 633 | 0.1863 | −0.4741 | −0.9904 | 1.8441 | 2.0613 | −0.5917 | −0.1769 |
| GI_34452701-S | ANKH | H sapiens ankylosis, progressive homolog (mouse; ANKH), mRNA | 56172 | −0.1405 | −0.3468 | −0.3980 | 1.9032 | 2.6121 | 0.1362 | 0.2076 |
| GI_37548655-S | OLFML2B | H sapiens olfactomedin-like 2B (OLFML2B) mRNA | 25903 | −0.1088 | −0.0092 | −0.1600 | 1.5639 | 1.6795 | −0.5550 | 0.1048 |
| GI_40317614-A | TMEPAI | H sapiens transmembrane, prostate androgen induced RNA (TMEPAI), transcript variant 1, mRNA | 56937 | −0.4838 | 0.2304 | 0.0321 | 2.3013 | 2.3872 | −0.1680 | −0.0132 |
| GI_41327755-S | ADAMTS4 | H sapiens a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 (ADAMTS4), mRNA | 9507 | −0.7378 | 0.3704 | 0.2196 | 2.1222 | 2.4524 | −0.2427 | −0.1231 |
| GI_32130529-A | CMTM3 | H sapiens CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), mRNA | 123920 | −0.0054 | 0.0109 | −0.1266 | 1.0540 | 1.7720 | −0.3590 | 0.0973 |
| GI_42716296-S | CLU | H sapiens clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J; CLU), transcript variant 1, mRNA | 1191 | −0.0165 | −0.4015 | −0.6002 | 1.8815 | 1.7451 | −0.1837 | −0.0081 |
| GI_4507398-S | TCF4 | H sapiens transcription factor 4 (TCF4), mRNA | 6925 | 0.4581 | −0.6464 | −0.8820 | 1.4544 | 1.8483 | −0.2636 | −0.0804 |
| GI_4507464-S | TGFB3 | H sapiens transforming growth factor, beta 3 (TGFB3), mRNA | 7043 | −0.0634 | 0.1799 | 0.3213 | 3.2386 | 3.2736 | −0.3335 | −0.4560 |
| GI_4755145-S | AEBP1 | H sapiens AE binding protein 1 (AEBP1), mRNA | 165 | 0.2491 | −1.5791 | −1.6139 | 1.9207 | 2.0746 | −0.3012 | 0.0292 |
| GI_5031970-S | LRRC17 | H sapiens leucine rich repeat containing 17 (LRRC17), mRNA | 10234 | 0.5369 | −0.0269 | 0.0042 | 2.6441 | 3.7125 | −2.4749 | −0.3653 |
| GI_41582237-A | ISLR | H sapiens immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1, mRNA | 3671 | 0.1956 | −0.8433 | −1.2155 | 2.6252 | 2.2217 | −0.7244 | −0.0703 |
| Osteogenic markers | ||||||||||
| GI_31324542-S | CAMK2N1 | H sapiens calcium/calmodulin-dependent protein kinase II (CaMKIINalpha), mRNA | 55450 | −0.22461 | −0.4192 | −0.34172 | −0.14237 | 0.118001 | 1.729828 | 1.220218 |
| GI_32895369-I | COL8A1 | H sapiens collagen, type VIII, alpha 1 (COL8A1), transcript variant 1, mRNA | 1295 | −0.44275 | 0.120977 | 0.2237 | −0.03571 | −0.64901 | 1.696772 | 1.708998 |
| GI_34335231-S | CKB | H sapiens creatine kinase, brain (CKB), mRNA | 1152 | −0.10611 | 0.06397 | 0.009828 | −1.00722 | −0.69708 | 1.666986 | 1.155259 |
| GI_4503056-S | CRYAB | H sapiens crystallin, alpha B (CRYAB), mRNA | 1410 | −0.54266 | 0.029788 | 0.42692 | −1.25125 | −1.56576 | 1.890013 | 1.347231 |
| GI_7110718-S | DKK1 | H sapiens dickkopf homolog 1 (Xenopus laevis; DKK1), mRNA | 22943 | 0.341599 | −0.41979 | −0.34115 | −1.46302 | −1.35283 | 1.528545 | 1.629448 |
| Probe ID . | Gene symbol . | Description . | Entrez Gene ID . | Sample ID . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| M1 . | A07 . | A14 . | C07 . | C14 . | O07 . | O14 . | ||||
| MSC markers | ||||||||||
| GI_21264358-I | MASP1 | Homo sapiens mannan-binding lectin serine protease 1 (C4/C2 activating component of Ra-reactive factor) (MASP1), transcript variant 2, mRNA | 5648 | 2.1247 | −0.1619 | 0.0000 | −0.9388 | 0.0000 | 0.2726 | 0.0000 |
| GI_5803016-S | FOSB | H sapiens FBJ murine osteosarcoma viral oncogene homolog B (FOSB), mRNA | 2354 | 3.0524 | −0.3024 | 1.8793 | 0.5454 | −1.7003 | −1.1363 | −0.0643 |
| Adipogenic markers | ||||||||||
| GI_12669906-S | IGFBP6 | H sapiens acyl-CoA synthetase long-chain family member 1 (ACSL1), mRNA | 2180 | −0.1096 | 1.3134 | 1.8922 | −0.1455 | −0.2354 | 0.0038 | 0.0985 |
| GI_20070269-S | G0S2 | H sapiens putative lymphocyte G0/G1 switch gene (G0S2), mRNA | 50486 | −0.4908 | 2.8306 | 3.2601 | 0.4378 | 0.8131 | −0.7489 | −0.5906 |
| GI_22027484-S | >RASD1 | H sapiens RAS, dexamethasone-induced 1 (RASD1), mRNA | 51655 | 0.8445 | 2.2295 | 1.7099 | −0.7705 | −3.3403 | −0.3931 | −0.1897 |
| GI_31377551-S | RAP2A | H sapiens RAP2A, member of RAS oncogene family (RAP2A), mRNA | 5911 | −0.2610 | 1.9745 | 2.3584 | 0.0316 | −0.7797 | −0.1838 | −0.0538 |
| GI_34222182-S | DDIT4L | H sapiens DNA-damage-inducible transcript 4-like (DDIT4L), mRNA | 115265 | −0.8135 | 1.9974 | 2.2798 | 0.6702 | 0.3898 | −2.5613 | −0.3804 |
| GI_45446741-S | AKR1C2 | H sapiens aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III) (AKR1C2), transcript variant 1, mRNA | 1646 | −0.3467 | 2.1801 | 2.4928 | 0.1962 | −0.0946 | −0.4847 | 0.0891 |
| Chondrogenic markers | ||||||||||
| GI_13324676-S | ENPP1 | H sapiens ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), mRNA | 5167 | 0.2188 | −0.7887 | −0.5631 | 1.9896 | 3.0516 | −1.1652 | −0.4323 |
| GI_17981693-A | CDKN2B | H sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1, mRNA | 1030 | 0.3149 | −0.0605 | −0.0050 | 1.8528 | 1.8740 | −0.8466 | −0.9401 |
| GI_19923197-S | RUNX1 | H sapiens runt-related transcription factor 1 (acute myeloid leukemia 1; aml1 oncogene; RUNX1), mRNA | 861 | 0.0426 | 0.0033 | −0.0123 | 1.6058 | 2.0494 | −0.7632 | −0.3739 |
| GI_23111004-S | MFAP4 | H sapiens microfibrillar-associated protein 4 (MFAP4), mRNA | 4239 | −0.2909 | −0.5414 | −1.7318 | 2.1228 | 1.7113 | 0.0428 | 0.6477 |
| GI_24430217-S | IL11 | H sapiens interleukin 11 (IL11), mRNA | 3589 | 0.4145 | −1.0965 | −0.7898 | 2.0906 | 2.4231 | −1.7893 | −1.0528 |
| GI_24475846-S | IVNS1ABP | H sapiens influenza virus NS1A binding protein (IVNS1ABP), mRNA | 10625 | −0.2214 | −0.4830 | −0.4939 | 1.4905 | 2.2335 | 0.4409 | 0.3169 |
| GI_29568085-S | SDC1 | H sapiens syndecan 1 (SDC1), mRNA | 6382 | 0.6379 | −1.7792 | −1.7169 | 2.0289 | 2.5978 | −1.7140 | −1.2265 |
| GI_32964829-S | COL8A2 | H sapiens collagen, type VIII, alpha 2 (COL8A2), mRNA | 1296 | −0.4979 | −0.1827 | −0.1771 | 2.3983 | 1.1285 | −0.1501 | 0.5740 |
| GI_34304351-S | BGN | H sapiens biglycan (BGN), mRNA | 633 | 0.1863 | −0.4741 | −0.9904 | 1.8441 | 2.0613 | −0.5917 | −0.1769 |
| GI_34452701-S | ANKH | H sapiens ankylosis, progressive homolog (mouse; ANKH), mRNA | 56172 | −0.1405 | −0.3468 | −0.3980 | 1.9032 | 2.6121 | 0.1362 | 0.2076 |
| GI_37548655-S | OLFML2B | H sapiens olfactomedin-like 2B (OLFML2B) mRNA | 25903 | −0.1088 | −0.0092 | −0.1600 | 1.5639 | 1.6795 | −0.5550 | 0.1048 |
| GI_40317614-A | TMEPAI | H sapiens transmembrane, prostate androgen induced RNA (TMEPAI), transcript variant 1, mRNA | 56937 | −0.4838 | 0.2304 | 0.0321 | 2.3013 | 2.3872 | −0.1680 | −0.0132 |
| GI_41327755-S | ADAMTS4 | H sapiens a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 (ADAMTS4), mRNA | 9507 | −0.7378 | 0.3704 | 0.2196 | 2.1222 | 2.4524 | −0.2427 | −0.1231 |
| GI_32130529-A | CMTM3 | H sapiens CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), mRNA | 123920 | −0.0054 | 0.0109 | −0.1266 | 1.0540 | 1.7720 | −0.3590 | 0.0973 |
| GI_42716296-S | CLU | H sapiens clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J; CLU), transcript variant 1, mRNA | 1191 | −0.0165 | −0.4015 | −0.6002 | 1.8815 | 1.7451 | −0.1837 | −0.0081 |
| GI_4507398-S | TCF4 | H sapiens transcription factor 4 (TCF4), mRNA | 6925 | 0.4581 | −0.6464 | −0.8820 | 1.4544 | 1.8483 | −0.2636 | −0.0804 |
| GI_4507464-S | TGFB3 | H sapiens transforming growth factor, beta 3 (TGFB3), mRNA | 7043 | −0.0634 | 0.1799 | 0.3213 | 3.2386 | 3.2736 | −0.3335 | −0.4560 |
| GI_4755145-S | AEBP1 | H sapiens AE binding protein 1 (AEBP1), mRNA | 165 | 0.2491 | −1.5791 | −1.6139 | 1.9207 | 2.0746 | −0.3012 | 0.0292 |
| GI_5031970-S | LRRC17 | H sapiens leucine rich repeat containing 17 (LRRC17), mRNA | 10234 | 0.5369 | −0.0269 | 0.0042 | 2.6441 | 3.7125 | −2.4749 | −0.3653 |
| GI_41582237-A | ISLR | H sapiens immunoglobulin superfamily containing leucine-rich repeat (ISLR), transcript variant 1, mRNA | 3671 | 0.1956 | −0.8433 | −1.2155 | 2.6252 | 2.2217 | −0.7244 | −0.0703 |
| Osteogenic markers | ||||||||||
| GI_31324542-S | CAMK2N1 | H sapiens calcium/calmodulin-dependent protein kinase II (CaMKIINalpha), mRNA | 55450 | −0.22461 | −0.4192 | −0.34172 | −0.14237 | 0.118001 | 1.729828 | 1.220218 |
| GI_32895369-I | COL8A1 | H sapiens collagen, type VIII, alpha 1 (COL8A1), transcript variant 1, mRNA | 1295 | −0.44275 | 0.120977 | 0.2237 | −0.03571 | −0.64901 | 1.696772 | 1.708998 |
| GI_34335231-S | CKB | H sapiens creatine kinase, brain (CKB), mRNA | 1152 | −0.10611 | 0.06397 | 0.009828 | −1.00722 | −0.69708 | 1.666986 | 1.155259 |
| GI_4503056-S | CRYAB | H sapiens crystallin, alpha B (CRYAB), mRNA | 1410 | −0.54266 | 0.029788 | 0.42692 | −1.25125 | −1.56576 | 1.890013 | 1.347231 |
| GI_7110718-S | DKK1 | H sapiens dickkopf homolog 1 (Xenopus laevis; DKK1), mRNA | 22943 | 0.341599 | −0.41979 | −0.34115 | −1.46302 | −1.35283 | 1.528545 | 1.629448 |
Genes with lineage-specific expression as shown in Figure 1. These putative markers were identified from ANOVA and post hoc analysis. All genes shown in this table have MSI more than 1000. Data are the normalized log intensity values for this analysis since it normalizes the signal in multiple replicates and accounts for variations in hybridization, scanning, and so on. This enables a direct comparison of expression across multiple samples carried out on different arrays, which is not always possible with MSI values.