MSCs from 3 donors at passage 3 and their progeny were used in this study. Samples on the humanRef-8v1 chip were organized such that replicate samples were on different chips. Each chip contained samples of all undifferentiated MSCs and their differentiated progeny, but samples from day 7 and day 14 were on separate chips. In total 6 replicates were hybridized for each sample. Each replicate was hybridized on a separate chip. Having each replicate on a different chip averages factors affecting the microarray signal such as hybridization conditions, scanning conditions, etcetera, and reduce variation from these sources in the final dataset. This design also serves as a good internal control for assessing the reproducibility of the arrays. Undifferentiated MSCs cultured for 7 and 14 days, respectively, were used to compare their transcriptome with their differentiated progeny at these time points.

— indicates not applicable.