Quantitation of CX3CL1 in brain and serum of healthy mice
| Tissue/genotype . | Cx3cr1+/+ . | Cx3cr1−/− . | Fold change . |
|---|---|---|---|
| Brain | |||
| Protein, pg* | 1500 ± 800 | 48000 ± 300† | > 30 |
| mRNA, fg | 44 ± 10 | 43 ± 10 | 1 |
| Blood, protein, pg* | 780 ± 200 | 191000 ± 80000‡ | > 300 |
| Tissue/genotype . | Cx3cr1+/+ . | Cx3cr1−/− . | Fold change . |
|---|---|---|---|
| Brain | |||
| Protein, pg* | 1500 ± 800 | 48000 ± 300† | > 30 |
| mRNA, fg | 44 ± 10 | 43 ± 10 | 1 |
| Blood, protein, pg* | 780 ± 200 | 191000 ± 80000‡ | > 300 |
Soluble CX3CL1 was measured by ELISA in brain aqueous homogenates (n = 4 mice per group) and serum (n = 9 mice per group).
Data represent the mean plus or minus standard deviation (SD) in pg/mg protein. Brain and circulating levels were significantly higher in Cx3cr1−/− mice compared with wild-type mice.
P < .001,
P = .003. CX3CL1 message was quantified by real time RT-PCR, showing no statistical significance in brain mRNA expression; n = 4 mice per group.