Details of antibodies used
| Antibody . | Clone . | Source . | Pretreatment . | Primary Ab dilution . |
|---|---|---|---|---|
| CD3 | PS1 | Vision Biosystems | Pressure cooker microwave | 1/100 |
| CD4 | 1F6 | Vector | Pressure cooker microwave | 1/20 |
| CD7 | CD7–272 | Vision Biosystems | Pressure cooker microwave | 1/50 |
| CD8 | CD8/144B | Cell Marque | Pressure cooker microwave | 1/25 |
| CD10 | 56C6 | Cell Marque | Pressure cooker microwave | 1/25 |
| CD20 | L26 | Vector | Pressure cooker microwave | 1/200 |
| CD21 | 2G9 | Vector | Pressure cooker microwave | 1/10 |
| CD68 | 514H12 | Vision Biosystems | Trypsin | 1/50 |
| Antibody . | Clone . | Source . | Pretreatment . | Primary Ab dilution . |
|---|---|---|---|---|
| CD3 | PS1 | Vision Biosystems | Pressure cooker microwave | 1/100 |
| CD4 | 1F6 | Vector | Pressure cooker microwave | 1/20 |
| CD7 | CD7–272 | Vision Biosystems | Pressure cooker microwave | 1/50 |
| CD8 | CD8/144B | Cell Marque | Pressure cooker microwave | 1/25 |
| CD10 | 56C6 | Cell Marque | Pressure cooker microwave | 1/25 |
| CD20 | L26 | Vector | Pressure cooker microwave | 1/200 |
| CD21 | 2G9 | Vector | Pressure cooker microwave | 1/10 |
| CD68 | 514H12 | Vision Biosystems | Trypsin | 1/50 |
All antibodies were detected with Envision detection kit supplied by DAKO. Locations of suppliers: Vision Biosystems, Newcastle, United Kingdom; Cell Marque, Rocklin, CA; Vector, Burlingame, CA.