Effect of etsrp overexpression and knockdown on pu.1 expression at the 8- to 10-somite stages
| . | Normal or slightly distorted pu.1 pattern . | Normal endogenous and ectopic pu.1 expression . | Reduced or absent pu.1 expression . | Reduced or absent endogenous and ectopic pu.1 expression . |
|---|---|---|---|---|
| Control (n > 50) | 100 | 0 | 0 | 0 |
| etsrp MO (n = 40) | 5 ± 5 | 0 | 95 ± 5 | 0 |
| etsrp RNA (n = 57) | 24 ± 11 | 16 ± 1 | 9 ± 9 | 51 ± 19 |
| etsrpMO + RNA (n = 56) | 1 ± 1 | 0 | 27 ± 1 | 72 ± 2 |
| . | Normal or slightly distorted pu.1 pattern . | Normal endogenous and ectopic pu.1 expression . | Reduced or absent pu.1 expression . | Reduced or absent endogenous and ectopic pu.1 expression . |
|---|---|---|---|---|
| Control (n > 50) | 100 | 0 | 0 | 0 |
| etsrp MO (n = 40) | 5 ± 5 | 0 | 95 ± 5 | 0 |
| etsrp RNA (n = 57) | 24 ± 11 | 16 ± 1 | 9 ± 9 | 51 ± 19 |
| etsrpMO + RNA (n = 56) | 1 ± 1 | 0 | 27 ± 1 | 72 ± 2 |
Etsrp RNA causes ectopic pu.1 expression in control embryos and etsrp morphants and also results in the reduction of endogenous etsrp expression. Results are percentage averages from 2 independent experiments.