Evolution of the Ly49I repertoire in NK cells over time
| Days after pulsing with BrdU* . | % BrdU+ NK cells in WT mice† . | % BrdU+ NK cells in BCAP−/− mice† . | % Ly49I+ NK cells within BrdU+ cells of WT mice† . | % Ly49I+ NK cells within BrdU+ cells of BCAP−/− mice† . |
|---|---|---|---|---|
| Day 0 | 38.4 ± 12.6 | 38.7 ± 8.0 | 38.3 ± 2.6 | 31.8 ± 4.8 |
| Day 14 | 32.6 ± 6.7 | 36.8 ± 2.0 | 39.5 ± 5.0 | 38.7 ± 2.8 |
| Day 65 | 6.2 ± 1.9 | 8.9 ± 1.0 | 44.1 ± 9.3 | 54.7 ± 2.5 |
| Days after pulsing with BrdU* . | % BrdU+ NK cells in WT mice† . | % BrdU+ NK cells in BCAP−/− mice† . | % Ly49I+ NK cells within BrdU+ cells of WT mice† . | % Ly49I+ NK cells within BrdU+ cells of BCAP−/− mice† . |
|---|---|---|---|---|
| Day 0 | 38.4 ± 12.6 | 38.7 ± 8.0 | 38.3 ± 2.6 | 31.8 ± 4.8 |
| Day 14 | 32.6 ± 6.7 | 36.8 ± 2.0 | 39.5 ± 5.0 | 38.7 ± 2.8 |
| Day 65 | 6.2 ± 1.9 | 8.9 ± 1.0 | 44.1 ± 9.3 | 54.7 ± 2.5 |
Each data point represents the mean plus or minus SD from 3 mice.
Wild-type and BCAP−/− mice were pulsed with 5′-BrdU in drinking water for 20 days before day 0. Splenocytes were harvested on the indicated days after pulsing for analysis by flow cytometry.
NK cells were gated as NK1.1+CD3−. The percentage of self-recognizing NK cells was identified by costaining with anti-Ly49I.