Table 1

Cellular systems and stimuli used in this study

Effector cellsAntigen-specific trogocytosis
Redirected trogocytosis, Target: P815 cells (FcR+)
Target cellsAntigen recognizedFigures in current paperTriggering antibodyFigures in current paper
OT-I CD8+ T cells RMA or EL4 cells (H-2Kb+) SIINFEKL peptide (OVA257-264) 1,2,4,S1 2C11 Anti-CD3 mAb 4A,B 
DO11.10 CD4+ T cells A20 cells (I-Ad+) OVA323-339 1,4,S1 2C11 Anti-CD3 mAb 4A 
MD4 B cells J558L cells or HEK cells Membrane-bound hen egg lysozyme (mHEL) expressed by transfection 1,Figure 2,Figure 3,Figure 4–5,S1,S2 187.1 Anti-k mAb 4A,B 
MD4 B cells HEK transfected with mutated mHELK97A Mutant K97A mHEL (low affinity) 4C,S2   
3-83 B cells C3H splenocytes H-2k (high affinity) 4D,E   
3-83 B cells B6 splenocytes H-2b (low affinity) 4D,E   
3-83 B cells Balb/c splenocytes H-2d (no affinity) 4D,E   
1H11-34 T cell hybridoma MD4 B cells (I-Ed+) HEL107-116 5    
Effector cellsAntigen-specific trogocytosis
Redirected trogocytosis, Target: P815 cells (FcR+)
Target cellsAntigen recognizedFigures in current paperTriggering antibodyFigures in current paper
OT-I CD8+ T cells RMA or EL4 cells (H-2Kb+) SIINFEKL peptide (OVA257-264) 1,2,4,S1 2C11 Anti-CD3 mAb 4A,B 
DO11.10 CD4+ T cells A20 cells (I-Ad+) OVA323-339 1,4,S1 2C11 Anti-CD3 mAb 4A 
MD4 B cells J558L cells or HEK cells Membrane-bound hen egg lysozyme (mHEL) expressed by transfection 1,Figure 2,Figure 3,Figure 4–5,S1,S2 187.1 Anti-k mAb 4A,B 
MD4 B cells HEK transfected with mutated mHELK97A Mutant K97A mHEL (low affinity) 4C,S2   
3-83 B cells C3H splenocytes H-2k (high affinity) 4D,E   
3-83 B cells B6 splenocytes H-2b (low affinity) 4D,E   
3-83 B cells Balb/c splenocytes H-2d (no affinity) 4D,E   
1H11-34 T cell hybridoma MD4 B cells (I-Ed+) HEL107-116 5    
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