Table 1 Immunophenotype of the monocytic... | American Society of Hematology

Table 1

Immunophenotype of the monocytic cells and tumor-infiltrating CD11b⁺Gr-1⁺ cell subpopulations

	Antigen presentation				Adhesion molecules						Other
	MHC Class I	MHC Class II	CD80	CD86	CD11a	CD11b	CD11c	CD29	CD43	CD62L	CD115	TNFα	ScaI	F4/80	CD24	Ly6C
Ly-6C^hi “inflammatory” monocytes	+++	−	++	ND
Thioglycollate-elicited macrophages	+++	+/−	++	++
Tumor-infiltrating CD11b⁺Gr-1^int/dull	+++	+/−	++	++
Tumor-infiltrating CD11b⁺ Gr-1^hi	++	−	+	−
Ly-6C^hi “inflammatory” monocytes					++	+++	−	+++	+++	+++
Thioglycollate-elicited macrophages					++	+++	+ or +++	+++	++	+
Tumor-infiltrating CD11b⁺Gr-1^int/dull					+++	+++	+	+++	++	+
Tumor-infiltrating CD11b⁺ Gr-1^hi					+++	+++	−	+	+++	−
Ly-6C^hi “inflammatory” monocytes											+++	ND	ND	++	ND	+++
Thioglycollate-elicited macrophages											+++	ND	+	++	+	++
Tumor-infiltrating CD11b⁺Gr-1^int/dull											+	+/−	++ or +++	++	++	++ or +++
Tumor-infiltrating CD11b⁺ Gr-1^hi											−	−	+	−	+++	+

	Antigen presentation				Adhesion molecules						Other
	MHC Class I	MHC Class II	CD80	CD86	CD11a	CD11b	CD11c	CD29	CD43	CD62L	CD115	TNFα	ScaI	F4/80	CD24	Ly6C
Ly-6C^hi “inflammatory” monocytes	+++	−	++	ND
Thioglycollate-elicited macrophages	+++	+/−	++	++
Tumor-infiltrating CD11b⁺Gr-1^int/dull	+++	+/−	++	++
Tumor-infiltrating CD11b⁺ Gr-1^hi	++	−	+	−
Ly-6C^hi “inflammatory” monocytes					++	+++	−	+++	+++	+++
Thioglycollate-elicited macrophages					++	+++	+ or +++	+++	++	+
Tumor-infiltrating CD11b⁺Gr-1^int/dull					+++	+++	+	+++	++	+
Tumor-infiltrating CD11b⁺ Gr-1^hi					+++	+++	−	+	+++	−
Ly-6C^hi “inflammatory” monocytes											+++	ND	ND	++	ND	+++
Thioglycollate-elicited macrophages											+++	ND	+	++	+	++
Tumor-infiltrating CD11b⁺Gr-1^int/dull											+	+/−	++ or +++	++	++	++ or +++
Tumor-infiltrating CD11b⁺ Gr-1^hi											−	−	+	−	+++	+

CD11b⁺Gr-1^intLy-6C^hi “inflammatory” monocytes were collected from peripheral blood of normal B6 mice. Thioglycollate-elicited macrophages were collected from peritoneal cavity 3 days after i.p. injection of 4% thioglycollate medium. Tumor-infiltrating CD11b⁺Gr-1^int/dull cells and CD11b⁺Gr-1^hi cells were analyzed at 10 days after tumor inoculation. − indicates no expression; +, ++, and +++, increasing amount of expression, +/−, + or ++, and ++ or +++, heterogeneous expression; ND, not determined.

View Large