Lentiviral transduction and engraftment of pigtailed macaque CD34+ cells
| Monkey* . | No. of CD34-enriched cells/kg ×106 before culture . | Purity of CD34-enriched cells, % . | No. of infused cells/kg ×106 . | MOI† . | Preinfusion transduction efficiency,‡ . | Days to ANC greater than 0.5 × 109/μL (500/μL) . | Days to platelet count greater than 2 × 1010/L (20 000/μL) . |
|---|---|---|---|---|---|---|---|
| J02043 (4.0 kg) | 20 | 82 | 4.6 | 10 | 4.3 (PCR) | 11 | 27 |
| J02370 (4.6 kg) | 30 | 97 | 11.7 | 10 | 30% (EGFP) | 10 | 35 |
| T04228 (3.6 kg) | 40 | 85 | 10.7 | 5 | 17.5% (EGFP) | 10 | 25 |
| Monkey* . | No. of CD34-enriched cells/kg ×106 before culture . | Purity of CD34-enriched cells, % . | No. of infused cells/kg ×106 . | MOI† . | Preinfusion transduction efficiency,‡ . | Days to ANC greater than 0.5 × 109/μL (500/μL) . | Days to platelet count greater than 2 × 1010/L (20 000/μL) . |
|---|---|---|---|---|---|---|---|
| J02043 (4.0 kg) | 20 | 82 | 4.6 | 10 | 4.3 (PCR) | 11 | 27 |
| J02370 (4.6 kg) | 30 | 97 | 11.7 | 10 | 30% (EGFP) | 10 | 35 |
| T04228 (3.6 kg) | 40 | 85 | 10.7 | 5 | 17.5% (EGFP) | 10 | 25 |
For all monkeys, the source of CD34+ cells was bone marrow.
Monkey and weight at time of transplantation in parentheses.
Multiplicity of infection based on titer determined by transduction of HT1080 cells.
Average proviral copy number in day-5 liquid cultures by real-time PCR (J02043) or percentage of fluorescence-positive cells assessed by flow cytometry for EGFP in liquid cultures on day 11 after transduction for vectors that contained EGFP (monkeys J02370 and T04228).