Table 1 Colony formation ability of... | American Society of Hematology

Table 1

Colony formation ability of fetal liver hematopoietic cells in response to various cytokines and growth factors is dependent on Jak2 and Stat5

Cytokine	WT GFP	WT cS5	Jak2^−/−-GFP	Jak2^−/−-cS5	EpoR^−/−-GFP	EpoR^−/−-cS5
Epo	392 ± 8	461 ± 5†	24 ± 8	264 ± 29†	35 ± 9	325 ± 57†
Epo + IL-3	75 ± 12	75 ± 20‡	3 ± 5	35 ± 9†	5 ± 5	48 ± 16†
GM-CSF	293 ± 26	432 ± 32†	56 ± 8	144 ± 29†	nd	nd
SCF	304 ± 8	352 ± 21*	109 ± 12	173 ± 20†	nd	nd
SCF + IL-3	448 ± 8	528 ± 28†	139 ± 12	219 ± 28†	nd	nd
SCF + IL-7	360 ± 14	408 ± 32‡	149 ± 20	216 ± 16†	nd	nd
None	0	0 ± 1	1	1 ± 1	1	0 ± 1

Cytokine	WT GFP	WT cS5	Jak2^−/−-GFP	Jak2^−/−-cS5	EpoR^−/−-GFP	EpoR^−/−-cS5
Epo	392 ± 8	461 ± 5†	24 ± 8	264 ± 29†	35 ± 9	325 ± 57†
Epo + IL-3	75 ± 12	75 ± 20‡	3 ± 5	35 ± 9†	5 ± 5	48 ± 16†
GM-CSF	293 ± 26	432 ± 32†	56 ± 8	144 ± 29†	nd	nd
SCF	304 ± 8	352 ± 21*	109 ± 12	173 ± 20†	nd	nd
SCF + IL-3	448 ± 8	528 ± 28†	139 ± 12	219 ± 28†	nd	nd
SCF + IL-7	360 ± 14	408 ± 32‡	149 ± 20	216 ± 16†	nd	nd
None	0	0 ± 1	1	1 ± 1	1	0 ± 1

SCF-induced colonies represent immature progenitors and mast cells, SCF + IL-3 and SCF + IL-7 induce colony formation of myeloid and lymphoid progenitors (data not shown).

nd indicates not determined.

P < .05.

†

P < .01.

‡

Not significant.

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