Characteristics of the SzS patients included in the study
| . | Patient no. . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | |
| Percentage SzS cells | 90 | 99 | 82 | 99 | 25 | 86 | 85 | 90 | 91 | 18 | 20 | 15 | 49 | 91 | 23 | 21 |
| CD4/CD8 ratio | 30 | 707 | 42 | 288 | 2 | 210 | 90 | 22.8 | 95 | 2.3 | 9.3 | 8 | ND | 115 | 9 | 40 |
| FasL sensitivity | – | – | – | – | – | – | – | – | – | + | + | + | + | + | + | + |
| Fas expression | – | – | + | + | + | – | + | – | + | + | + | + | + | + | + | + |
| TRAIL sensitivity | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| DR5 expression | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| cFLIP expression | ND | ND | ++ | ++ | ++ | ND | ++ | ND | ++ | ND | + | ND | ND | + | + | + |
| . | Patient no. . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | |
| Percentage SzS cells | 90 | 99 | 82 | 99 | 25 | 86 | 85 | 90 | 91 | 18 | 20 | 15 | 49 | 91 | 23 | 21 |
| CD4/CD8 ratio | 30 | 707 | 42 | 288 | 2 | 210 | 90 | 22.8 | 95 | 2.3 | 9.3 | 8 | ND | 115 | 9 | 40 |
| FasL sensitivity | – | – | – | – | – | – | – | – | – | + | + | + | + | + | + | + |
| Fas expression | – | – | + | + | + | – | + | – | + | + | + | + | + | + | + | + |
| TRAIL sensitivity | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| DR5 expression | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| cFLIP expression | ND | ND | ++ | ++ | ++ | ND | ++ | ND | ++ | ND | + | ND | ND | + | + | + |
Percentage of SzS cells was determined by flow cytometry or immunostaining of CD4+/CD26− cells in peripheral blood after cytospin. Ratios are calculated as follows: number of CD8/mL divided by number of CD4/mL of blood. CD4+ cells from patients with SzS were considered sensitive to FasL for <60% annexin-V-/PI-CD4+ cells by flow cytometry. Fas was considered to be significantly expressed when the FasΔMFI was >2 in CD4+ SzS cells.
ND indicates not determined; –, negative; +, positive/moderate; and ++, positive/high.