Results of cytogenetic and FISH analysis
| Case no. . | Cytogenetics . | FISH . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample/status . | Results . | Examined loci . | Other probes . | Newly identified aberrations . | ||||||||||
| IGH . | IGK . | IGL . | CCND1 . | CCND2 . | CCND3 . | p16 . | p27 . | RB1 . | TP53 . | |||||
| 1* | LN/D | 46, XY[10] | ||||||||||||
| BM/P | 44, XX, del(1)(p11p31), add(10)(p15),-11, -12,-14, -15,-20,-22,+4mar [3] | 2F | 2F | 2F | 2F | 2F | 2F | ND | ND | ND | ND | |||
| PB/P | 44, idem, t(4;9)(q13; q13)[1][cp3] | |||||||||||||
| 2* | S/P | 46-47, XY, del(3)(q21)[8], add(5)(p15),-6 [3], add(6) (q25)[8], der(14)t(7;14) (p11;p11),+mar [cp11] | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ||
| 3* | LN/P | 48, XY, t(2;12)(p11;p13),+3, +15 [6] | 2F | 1F1R1G (25%) | 2F | 2F | 1F1R1G (26%) | 2F | 1s (28%) | 2s | 2s | 2s | del(9)(p21p21) | |
| 4 | LN/D | 46, XY, del(2)(q31), t(9;11) (q34;q13)[5] | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ish der(9)t(9;11)(q34;q13) (CCND1+, RP5-901A4+) | |
| 5 | LN/P | No mitosis | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ||
| 6 | LN/D | 46, XY[5] | ||||||||||||
| PB/D | 45-47, XY,+3, del(4)(q31), del(5)(q21q33),-6[4], del(6) (q14q24), add(6)(q12)[2], +8, t(8;11) ((q21;q23),-9,-10, -12, add(14)(q32), der(15) t(10;15)(q21;q14),-17,+19, del(20)(p11), +1-2mar [cp6] | 1F1R1G (76%) | 2F | 2F | 2F | 2F | 2F | 1s (74%) | 2s | 1s (78%) | 1s (78%) | ish add(14)(q32)(3′IGH+, 5′IGH-, wcp6+, CCND3+, RP11-321B9+);ish del(20) (p11)(5′IGH+, CEP20+, RP11-329D4-, CTB- 530N10-, CTP-81F12+) | der(14)t(6;14)(p21;q32), der(20) t(14;20)(q32;p11), del(13)(q14q14) | |
| 7 | LN/D | No mitosis | 1F1R1G (80%) | 2F | 2F | 2F | 3F (30%) | 2F | 1s (80%) | 1s (34%) | 1s (78%) | 2s | nuc ish(MYCNx2),(3′IGHx2), (MYCN con 3′IGHx1); (RP11-282G6x2, RP11- 542H15x2)(RP11-282G6 sep RP11-542H15x1) | t(2;14)(p24;q32), del(9)(p21p21), del(12)(p13p13), del(13)(q14q14), |
| 8 | BM/D | 49, XY,+3, del(6)(q23q25), del(9)(p21), del(13) (q14q22), -15,+18,+add(18)(p11), +19 [5] | 1F1R1G (75%) | 2F | 2F | 2F | 2F | 2F | 1s (22%) | 2s | 1s (79%) | 2s | ish der(14)t(2;14)(p24;q32) (3′IGH+,5′IGH-, RP11- 417P24+, MYCN+, RP11 -282G6+); ish der(2)t(2;14) (p24;q32) (MYCN-, RP11- 282G6-, RP11-542H15+, 5′IGH+, RP11-417P24+); nuc ish (CCNE1x3) | t(2;14)(p24;q32) |
| Case no. . | Cytogenetics . | FISH . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample/status . | Results . | Examined loci . | Other probes . | Newly identified aberrations . | ||||||||||
| IGH . | IGK . | IGL . | CCND1 . | CCND2 . | CCND3 . | p16 . | p27 . | RB1 . | TP53 . | |||||
| 1* | LN/D | 46, XY[10] | ||||||||||||
| BM/P | 44, XX, del(1)(p11p31), add(10)(p15),-11, -12,-14, -15,-20,-22,+4mar [3] | 2F | 2F | 2F | 2F | 2F | 2F | ND | ND | ND | ND | |||
| PB/P | 44, idem, t(4;9)(q13; q13)[1][cp3] | |||||||||||||
| 2* | S/P | 46-47, XY, del(3)(q21)[8], add(5)(p15),-6 [3], add(6) (q25)[8], der(14)t(7;14) (p11;p11),+mar [cp11] | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ||
| 3* | LN/P | 48, XY, t(2;12)(p11;p13),+3, +15 [6] | 2F | 1F1R1G (25%) | 2F | 2F | 1F1R1G (26%) | 2F | 1s (28%) | 2s | 2s | 2s | del(9)(p21p21) | |
| 4 | LN/D | 46, XY, del(2)(q31), t(9;11) (q34;q13)[5] | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ish der(9)t(9;11)(q34;q13) (CCND1+, RP5-901A4+) | |
| 5 | LN/P | No mitosis | 2F | 2F | 2F | 2F | 2F | 2F | 2s | 2s | 2s | 2s | ||
| 6 | LN/D | 46, XY[5] | ||||||||||||
| PB/D | 45-47, XY,+3, del(4)(q31), del(5)(q21q33),-6[4], del(6) (q14q24), add(6)(q12)[2], +8, t(8;11) ((q21;q23),-9,-10, -12, add(14)(q32), der(15) t(10;15)(q21;q14),-17,+19, del(20)(p11), +1-2mar [cp6] | 1F1R1G (76%) | 2F | 2F | 2F | 2F | 2F | 1s (74%) | 2s | 1s (78%) | 1s (78%) | ish add(14)(q32)(3′IGH+, 5′IGH-, wcp6+, CCND3+, RP11-321B9+);ish del(20) (p11)(5′IGH+, CEP20+, RP11-329D4-, CTB- 530N10-, CTP-81F12+) | der(14)t(6;14)(p21;q32), der(20) t(14;20)(q32;p11), del(13)(q14q14) | |
| 7 | LN/D | No mitosis | 1F1R1G (80%) | 2F | 2F | 2F | 3F (30%) | 2F | 1s (80%) | 1s (34%) | 1s (78%) | 2s | nuc ish(MYCNx2),(3′IGHx2), (MYCN con 3′IGHx1); (RP11-282G6x2, RP11- 542H15x2)(RP11-282G6 sep RP11-542H15x1) | t(2;14)(p24;q32), del(9)(p21p21), del(12)(p13p13), del(13)(q14q14), |
| 8 | BM/D | 49, XY,+3, del(6)(q23q25), del(9)(p21), del(13) (q14q22), -15,+18,+add(18)(p11), +19 [5] | 1F1R1G (75%) | 2F | 2F | 2F | 2F | 2F | 1s (22%) | 2s | 1s (79%) | 2s | ish der(14)t(2;14)(p24;q32) (3′IGH+,5′IGH-, RP11- 417P24+, MYCN+, RP11 -282G6+); ish der(2)t(2;14) (p24;q32) (MYCN-, RP11- 282G6-, RP11-542H15+, 5′IGH+, RP11-417P24+); nuc ish (CCNE1x3) | t(2;14)(p24;q32) |
LN, lymph node; D, diagnosis; BM, bone marrow; P, progression; F, fused signal; ND, not done; PB, peripheral blood; S, spleen; s, signal; R, red signal; G, green signal.
Previously published case.2