Rate constants of proteolysis at R306 or R506 in recombinant factor Va by APC
| FVa (R306Q/R769Q) . | k506, mol−1 sec−1 . | Fold-stimulation . | FVa (R506Q/R679Q) . | k306, mol−1 sec−1 . | Fold-stimulation . |
|---|---|---|---|---|---|
| APC | 1.89 × 108 | — | APC | 2.41 × 106 | — |
| APC plus protein S | 2.40 × 108 | 1.27 | APC + protein S | 7.58 × 107 | 31 |
| APC plus protein S-C4BP | 6.72 × 107 | 0.36 | APC + protein S-C4BP | 3.39 × 107 | 14 |
| FVa (R306Q/R769Q) . | k506, mol−1 sec−1 . | Fold-stimulation . | FVa (R506Q/R679Q) . | k306, mol−1 sec−1 . | Fold-stimulation . |
|---|---|---|---|---|---|
| APC | 1.89 × 108 | — | APC | 2.41 × 106 | — |
| APC plus protein S | 2.40 × 108 | 1.27 | APC + protein S | 7.58 × 107 | 31 |
| APC plus protein S-C4BP | 6.72 × 107 | 0.36 | APC + protein S-C4BP | 3.39 × 107 | 14 |
Concentrations of protein S and protein S-C4BP were 200 nM. Average rate constants from 2 separate experiments were determined as described.16
— indicates no data.