Normal and 5q− clonal LTC-IC activity of 5q− CD34+CD38+Thy-1− and CD34+CD38−Thy-1+ cells
| Patient no. . | LTC-CFC/1000 cells . | |||
|---|---|---|---|---|
| CD34+CD38+Thy-1− cells . | CD34+CD38−Thy-1+ cells . | |||
| Normal . | 5q deleted . | Normal . | 5q deleted . | |
| 1 | 0 | 0 | 0 | 29 |
| 2 | 0 | 0 | 0 | 0 |
| 4 | 0 | 0 | 0 | 0 |
| 6 | 0 | 0 | 0 | 51 |
| Normal mean, n=5 | 0 | 0 | 1838 | 0 |
| Patient no. . | LTC-CFC/1000 cells . | |||
|---|---|---|---|---|
| CD34+CD38+Thy-1− cells . | CD34+CD38−Thy-1+ cells . | |||
| Normal . | 5q deleted . | Normal . | 5q deleted . | |
| 1 | 0 | 0 | 0 | 29 |
| 2 | 0 | 0 | 0 | 0 |
| 4 | 0 | 0 | 0 | 0 |
| 6 | 0 | 0 | 0 | 51 |
| Normal mean, n=5 | 0 | 0 | 1838 | 0 |
Six-week LTC-CFC activity of CD34+CD38−Thy-1+ (75-750 cells/well) and CD34+CD38+Thy-1− (750 cells/well) cells from healthy controls and 5q− syndrome patients were evaluated as described in “LTC-IC assay.” After 6 weeks of coculture, the number of CFCs produced after an additional 2 weeks in methylcellulose were counted. Results are the means of 3 to 6 replicate wells from each patient and cell population. No LTC-CFCs were derived from normal or 5q− CD34+CD38+Thy-1− candidate progenitor cells.