Efficiency of colony formation in soft agar
| Cells* . | Colony-forming efficiency, % . | Average size of colonies, μm† . |
|---|---|---|
| B5-EV1 | 0.7 ± 0.5 | 62.6 ± 1.5 |
| B5-NIK#1 | 23.2 ± 2.0‡ | 236.2 ± 12.6‡ |
| B5-NIK#2 | 18.9 ± 2.4‡ | 184.1 ± 19.8‡ |
| B5-kd-NIK#1 | 1.5 ± 0.3 | 63.1 ± 1.4 |
| B5-kd-NIK#2 | 1.3 ± 0.1 | 62.8 ± 1.8 |
| h12-EV1 | 1.2 ± 0.3 | 60.5 ± 0.0 |
| h12-NIK#1 | 12.8 ± 1.7‡ | 146.9 ± 4.6‡ |
| h12-NIK#2 | 17.7 ± 1.7‡ | 154.9 ± 5.6‡ |
| h12-kd-NIK#1 | 1.4 ± 1.0 | 61.5 ± 2.1 |
| h12-kd-NIK#2 | 1.5 ± 0.4 | 62.5 ± 4.7 |
| B5-EV1-EV2 | 1.2 ± 0.3 | 61.8 ± 1.1 |
| B5-NIK#1-EV2 | 21.1 ± 1.0‡ | 193.8 ± 3.7‡ |
| B5-NIK#2-EV2 | 14.3 ± 1.0‡ | 150.4 ± 8.7‡ |
| h12-EV1-EV2 | 1.5 ± 0.7 | 60.8 ± 0.4 |
| h12-NIK#1-EV2 | 12.3 ± 1.7‡ | 119.4 ± 5.6‡ |
| h12-NIK#2-EV2 | 14.0 ± 1.8‡ | 160.3 ± 7.2‡ |
| B5-EV1-SR-IκBα | 1.5 ± 0.0 | 61.7 ± 0.5 |
| B5-NIK#1-SR-IκBα | 3.4 ± 0.0 | 64.8 ± 1.1 |
| B5-NIK#2-SR-IκBα | 3.9 ± 0.1 | 63.3 ± 0.4 |
| h12-EV1-SR-IκBα | 1.7 ± 1.0 | 61.3 ± 0.4 |
| h12-NIK#1-SR-IκBα | 2.7 ± 0.3 | 62.3 ± 0.1 |
| h12-NIK#2-SR-IκBα | 3.4 ± 1.4 | 61.4 ± 0.2 |
| Cells* . | Colony-forming efficiency, % . | Average size of colonies, μm† . |
|---|---|---|
| B5-EV1 | 0.7 ± 0.5 | 62.6 ± 1.5 |
| B5-NIK#1 | 23.2 ± 2.0‡ | 236.2 ± 12.6‡ |
| B5-NIK#2 | 18.9 ± 2.4‡ | 184.1 ± 19.8‡ |
| B5-kd-NIK#1 | 1.5 ± 0.3 | 63.1 ± 1.4 |
| B5-kd-NIK#2 | 1.3 ± 0.1 | 62.8 ± 1.8 |
| h12-EV1 | 1.2 ± 0.3 | 60.5 ± 0.0 |
| h12-NIK#1 | 12.8 ± 1.7‡ | 146.9 ± 4.6‡ |
| h12-NIK#2 | 17.7 ± 1.7‡ | 154.9 ± 5.6‡ |
| h12-kd-NIK#1 | 1.4 ± 1.0 | 61.5 ± 2.1 |
| h12-kd-NIK#2 | 1.5 ± 0.4 | 62.5 ± 4.7 |
| B5-EV1-EV2 | 1.2 ± 0.3 | 61.8 ± 1.1 |
| B5-NIK#1-EV2 | 21.1 ± 1.0‡ | 193.8 ± 3.7‡ |
| B5-NIK#2-EV2 | 14.3 ± 1.0‡ | 150.4 ± 8.7‡ |
| h12-EV1-EV2 | 1.5 ± 0.7 | 60.8 ± 0.4 |
| h12-NIK#1-EV2 | 12.3 ± 1.7‡ | 119.4 ± 5.6‡ |
| h12-NIK#2-EV2 | 14.0 ± 1.8‡ | 160.3 ± 7.2‡ |
| B5-EV1-SR-IκBα | 1.5 ± 0.0 | 61.7 ± 0.5 |
| B5-NIK#1-SR-IκBα | 3.4 ± 0.0 | 64.8 ± 1.1 |
| B5-NIK#2-SR-IκBα | 3.9 ± 0.1 | 63.3 ± 0.4 |
| h12-EV1-SR-IκBα | 1.7 ± 1.0 | 61.3 ± 0.4 |
| h12-NIK#1-SR-IκBα | 2.7 ± 0.3 | 62.3 ± 0.1 |
| h12-NIK#2-SR-IκBα | 3.4 ± 1.4 | 61.4 ± 0.2 |
kd-NIK indicates catalytically inactive NIK; SR, super-repressor; EV1, empty vector for NIK or kd-NIK; and EV2, empty vector for SR-IκBα.
Cells were inoculated in 0.33% soft agar and cultured for 3 weeks.
Colonies larger than 60 μm were counted as positive. The sizes of more than 100 positive colonies were averaged.
P < .05 vs B5-EV1.