Expression of Epo-GFP transgenes and GATA factors
| Tissue . | Epo-GFP transgene . | GATA factors . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type . | GATA mutant . | Gata2 . | Gata3 . | GATA-4 . | ||||||
| N . | A . | N . | A . | N . | A . | N . | A . | N . | A . | |
| Kidney | ||||||||||
| Peritubular interstitial | − | ++ | − | ++ | + | + | − | − | + | + |
| Distal/collecting tubules | − | − | ++ | ++ | ++ | ++ | ++ | ++ | − | − |
| Liver | ||||||||||
| Hepatocyte | − | + | − | + | − | − | − | − | + | + |
| Bile duct epithelium | − | − | ± | ± | − | − | − | − | − | − |
| Lung: bronchial epithelium | − | − | ± | ± | − | − | − | − | − | − |
| Thymus: thymic medullary epithelium | − | − | ± | ± | − | − | − | − | − | − |
| Tissue . | Epo-GFP transgene . | GATA factors . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type . | GATA mutant . | Gata2 . | Gata3 . | GATA-4 . | ||||||
| N . | A . | N . | A . | N . | A . | N . | A . | N . | A . | |
| Kidney | ||||||||||
| Peritubular interstitial | − | ++ | − | ++ | + | + | − | − | + | + |
| Distal/collecting tubules | − | − | ++ | ++ | ++ | ++ | ++ | ++ | − | − |
| Liver | ||||||||||
| Hepatocyte | − | + | − | + | − | − | − | − | + | + |
| Bile duct epithelium | − | − | ± | ± | − | − | − | − | − | − |
| Lung: bronchial epithelium | − | − | ± | ± | − | − | − | − | − | − |
| Thymus: thymic medullary epithelium | − | − | ± | ± | − | − | − | − | − | − |
The GFP expression of wild-type (wt-Epo-GFP) and GATA mutant (m1-Epo-GFP and m2-Epo-GFP) transgenic mice was examined under normal (N) and anemic (A) conditions. Gata2 and Gata3 expression was examined using GFP and LacZ knock-in mouse lines, respectively. Anti–GATA-4 antibody was used for detecting endogenous GATA-4–expressing cells.
− indicates negative; ++, positive with a high expression level; +, positive in all lines examined; and ±, positive in lines 1A, 1B, and 2A, but negative in lines 1C, 2B, and 2C.