Summary of the vascular defects in crlr morphant embryos
| Lack of blood circulation . | Alterations in ISV development . | Alterations in trunk DA development . | Interruption at the anterior DA bifurcation . | crlr-MO1, % of injected embryos± SD . | crlr-MO2, % of injected embryos± SD . |
|---|---|---|---|---|---|
| − | − | − | − | 14 ± 2 | 35 ± 7 |
| + | − | + | − | 21 ± 6 | 21 ± 4 |
| + | − | + | + | 20 ± 4 | 26 ± 10 |
| + | + | + | + | 45 ± 4 | 9 ± 1 |
| Lack of blood circulation . | Alterations in ISV development . | Alterations in trunk DA development . | Interruption at the anterior DA bifurcation . | crlr-MO1, % of injected embryos± SD . | crlr-MO2, % of injected embryos± SD . |
|---|---|---|---|---|---|
| − | − | − | − | 14 ± 2 | 35 ± 7 |
| + | − | + | − | 21 ± 6 | 21 ± 4 |
| + | − | + | + | 20 ± 4 | 26 ± 10 |
| + | + | + | + | 45 ± 4 | 9 ± 1 |
One- to 4-cell stage zebrafish VEGFR2:G-RCFP embryos were injected with 0.8 pmol crlr-MO1 (n = 34) or crlr-MO2 (n = 25), and vascular defects were assessed at 28 hpf. Data are expressed as percentage of morphants showing the indicated vascular defect(s). The results are the mean plus or minus SD of two independent experiments. No vascular defects were observed in std-MO–injected embryos (n = 32). − indicates absence of the phenotypic defect; +, presence of the phenotypic defect.